Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CD16 + CD32 antibody [EPR22345] (ab228971)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22345] to CD16 + CD32
Suitable for: WB, IHC-P
Reacts with: Mouse

Product name

Anti-CD16 + CD32 antibody [EPR22345]
Description

Rabbit monoclonal [EPR22345] to CD16 + CD32
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Mouse

WB
Mouse

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse spleen, liver and thymus lysate. His-tagged mouse CD16 recombinant protein(aa31-215) and His-tagged mouse CD32 recombinant protein (aa30-210). J774A.1 and DC2.4 whole cell lysate. IHC-P: Mouse liver and colon tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Mouse
WB	Mouse

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22345
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR22345] (ab228971)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD16 with ab228971 at 1/4,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on Kupffer cells and sinusoidal cells of mouse liver (PMID:26512139) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-CD16 + CD32 antibody [EPR22345] (ab228971)

All lanes : Anti-CD16 + CD32 antibody [EPR22345] (ab228971) at 1/1000 dilution

Lane 1 : Mouse spleen lysate
Lane 2 : Mouse thymus lysate
Lane 3 : Mouse liver lysate
Lane 4 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate
Lane 5 : DC2.4 (mouse dendritic cell) whole cell lysate
Lane 6 : bEnd.3 (mouse brain endothelioma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 29 kDa
Observed band size: 30-55 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lanes 1,2,3 and 6: 3 minutes; Lanes 4 and 5: 15 seconds.

Bands between 30-55 KD are likely to be glycosylated forms of CD16 and CD32 (PMID: 9378972, PMID: 19503602).

Negative control: bEnd.3 cell line (PMID: 23911392).
Western blot - Anti-CD16 + CD32 antibody [EPR22345] (ab228971)

All lanes : Anti-CD16 + CD32 antibody [EPR22345] (ab228971) at 1/1000 dilution

Lane 1 : His-tagged mouse CD16 recombinant protein (aa31-215), 20 ng
Lane 2 : His-tagged mouse CD32 recombinant protein (aa30-210), 20 ng

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 29 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?

Exposure time: 6 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR22345] (ab228971)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling CD16 with ab228971 at 1/4,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on stromal cells in mouse colon (PMID:26512139) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-CD16 + CD32 antibody [EPR22345] (ab228971)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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