Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD16 + CD32 antibody [EPR22345] (ab228971)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22345] to CD16 + CD32
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD16 + CD32 antibody [EPR22345]

  • Description

    Rabbit monoclonal [EPR22345] to CD16 + CD32

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse spleen, liver and thymus lysate. His-tagged mouse CD16 recombinant protein(aa31-215) and His-tagged mouse CD32 recombinant protein (aa30-210). J774A.1 and DC2.4 whole cell lysate. IHC-P: Mouse liver and colon tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR22345] (ab228971)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR22345] (ab228971)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD16 with ab228971 at 1/4,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on Kupffer cells and sinusoidal cells of mouse liver (PMID:26512139) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-CD16 + CD32 antibody [EPR22345] (ab228971)
    Western blot - Anti-CD16 + CD32 antibody [EPR22345] (ab228971)
    All lanes : Anti-CD16 + CD32 antibody [EPR22345] (ab228971) at 1/1000 dilution

    Lane 1 : Mouse spleen lysate
    Lane 2 : Mouse thymus lysate
    Lane 3 : Mouse liver lysate
    Lane 4 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate
    Lane 5 : DC2.4 (mouse dendritic cell) whole cell lysate
    Lane 6 : bEnd.3 (mouse brain endothelioma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 29 kDa
    Observed band size: 30-55 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1,2,3 and 6: 3 minutes; Lanes 4 and 5: 15 seconds.

    Bands between 30-55 KD are likely to be glycosylated forms of CD16 and CD32 (PMID: 9378972, PMID: 19503602).

    Negative control: bEnd.3 cell line (PMID: 23911392).

     

     

     

  • Western blot - Anti-CD16 + CD32 antibody [EPR22345] (ab228971)
    Western blot - Anti-CD16 + CD32 antibody [EPR22345] (ab228971)
    All lanes : Anti-CD16 + CD32 antibody [EPR22345] (ab228971) at 1/1000 dilution

    Lane 1 : His-tagged mouse CD16 recombinant protein (aa31-215), 20 ng
    Lane 2 : His-tagged mouse CD32 recombinant protein (aa30-210), 20 ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 29 kDa
    Observed band size: 21 kDa why is the actual band size different from the predicted?


    Exposure time: 6 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR22345] (ab228971)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR22345] (ab228971)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling CD16 with ab228971 at 1/4,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on stromal cells in mouse colon (PMID:26512139) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-CD16 + CD32 antibody [EPR22345] (ab228971)
    Anti-CD16 + CD32 antibody [EPR22345] (ab228971)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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