Human MAPK14 (p38) knockout HEK-293T cell line (ab255406)
Properties
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Number of cells
1 x 106 cells/vial, 1 mL -
Viability
~90% -
Adherent /Suspension
Adherent -
Tissue
Kidney -
Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12 -
Mycoplasma free
Yes -
Storage instructions
Shipped on Dry Ice. Store in liquid nitrogen. -
Storage buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether -
Purity
Immunogen affinity purified -
Research areas
Images
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Western blot - Human MAPK14 knockout HEK293T cell line (ab255406)All lanes : Anti-p38 alpha/MAPK14 antibody [EPR16878] (ab182453) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Wild-type HEK-293T cell lysate
Lane 4 : MAPK14 knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution
Predicted band size: 41 kDa
Additional bands at: 124 kDa (possible Loading Control)Lanes 1 - 4: Merged signal (red and green). Green - ab182453 observed at 40 kDa. Red - loading control, ab130007 observed at 125 kDa.
ab182453 was shown to react with p38 in wild-type HEK-293TT cells. Loss of signal was observed when knockout cell line ab255406 (knockout cell lysate ab263787) was used. Wild-type and p38 knockout samples were subjected to SDS-PAGE. ab182453 and Anti-Vinculin antibody [VIN-54] (ab130007) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Human MAPK14 knockout HEK293T cell line (ab255406)All lanes : Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Wild-type HEK-293T cell lysate
Lane 4 : MAPK14 knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution
Predicted band size: 41 kDa
Additional bands at: 124 kDa (possible Loading Control)Lanes 1 - 4: Merged signal (red and green). Green - ab32142 observed at 40 kDa. Red - loading control, ab130007 observed at 125 kDa.
ab32142 was shown to react with p38 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255406 (knockout cell lysate ab263787) was used. Wild-type and p38 knockout samples were subjected to SDS-PAGE. ab32142 and Anti-Vinculin antibody [VIN-54] (ab130007) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human MAPK14 knockout HEK293T cell line (ab255406)Allele-1: 1 bp insertion in exon 1
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Sanger Sequencing - Human MAPK14 knockout HEK293T cell line (ab255406)Allele-2: 11 bp deletion in exon 1.
