Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD16 + CD32 antibody [EPR22345] - BSA and Azide free (ab245199)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22345] to CD16 + CD32 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD16 + CD32 antibody [EPR22345] - BSA and Azide free

  • Description

    Rabbit monoclonal [EPR22345] to CD16 + CD32 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse spleen, liver and thymus lysate. His-tagged mouse CD16 recombinant protein(aa31-215) and His-tagged mouse CD32 recombinant protein (aa30-210). J774A.1 and DC2.4 whole cell lysate. IHC-P: Mouse liver and colon tissue.

  • General notes

    Ab245199 is the carrier-free version of ab228971. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab245199 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR22345] - BSA and Azide free (ab245199)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR22345] - BSA and Azide free (ab245199)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling CD16 with ab228971 at 1/4,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on stromal cells in mouse colon (PMID:26512139) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228971).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR22345] - BSA and Azide free (ab245199)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR22345] - BSA and Azide free (ab245199)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD16 with ab228971 at 1/4,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on Kupffer cells and sinusoidal cells of mouse liver (PMID:26512139) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228971).
  • Anti-CD16 + CD32 antibody [EPR22345] - BSA and Azide free (ab245199)
    Anti-CD16 + CD32 antibody [EPR22345] - BSA and Azide free (ab245199)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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