Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1 antibody [EPR22492-64] (ab252938)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22492-64] to KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1 antibody [EPR22492-64]

  • Description

    Rabbit monoclonal [EPR22492-64] to KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged human KIR2DL1 (aa1-245), His and LIF-tagged human KIR2DL2 (aa26-221), His-tagged human KIR2DL3 (aa1-245), His-tagged human KIR2DS1 (aa22-221) and His-tagged human KIR2DS2 recombinant proteins (aa22-221). NK-92 treated with 5-aza-2-deoxycytidine whole cell lysate. IHC-P: Human colon cancer, gastric cancer and endometrium tissue. ICC/IF: NK-92 cells treated with 5-aza-2-deoxycytidine. Flow Cyt: Human PBMC. IP: NK-92 treated with 5-aza-2-deoxycytidine, whole cell lysate

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22492-64

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-KIR2DL1 antibody [EPR22492-64] (ab252938)
    Immunocytochemistry/ Immunofluorescence - Anti-KIR2DL1 antibody [EPR22492-64] (ab252938)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell) cells labeling KIR2DL1 with ab252938 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in NK-92 cells treated with 5-aza-2-deoxycytidine(2µM) for 48hrs. The nuclear counter stain is DAPI (blue). Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red). The negative control is the secondary antibody only.

  • Western blot - Anti-KIR2DL1 antibody [EPR22492-64] (ab252938)
    Western blot - Anti-KIR2DL1 antibody [EPR22492-64] (ab252938)
    All lanes : Anti-KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1 antibody [EPR22492-64] (ab252938) at 1/1000 dilution

    Lane 1 : His-tagged human KIR2DL1 recombinant protein (aa1-245) 20 ng
    Lane 2 : His and LIF-tagged human KIR2DL2 recombinant protein (aa26-221) 20 ng
    Lane 3 : His-tagged human KIR2DL3 recombinant protein (aa1-245) 20 ng
    Lane 4 : His-tagged human KIR2DS1 recombinant protein (aa22-221) 20 ng
    Lane 5 : His-tagged human KIR2DS2 recombinant protein (aa22-221) 20 ng
    Lane 6 : His-tagged human KIR2DS3 recombinant protein (aa22-221) 20 ng
    Lane 7 : His-tagged human KIR2DS4 recombinant protein (aa22-221) 20 ng
    Lane 8 : His-tagged human KIR2DS5 recombinant protein (aa22-221) 20 ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 34, 38, 39 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-3: 15 secs; Lanes 4-8: 3 mins.

  • Western blot - Anti-KIR2DL1 antibody [EPR22492-64] (ab252938)
    Western blot - Anti-KIR2DL1 antibody [EPR22492-64] (ab252938)
    All lanes : Anti-KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1 antibody [EPR22492-64] (ab252938) at 1/1000 dilution

    Lane 1 : Untreated NK-92 (human malignant non-hodgkin's lymphoma natural killer cell) whole cell lysate
    Lane 2 : NK-92 treated with 2µM 5-aza-2-deoxycytidine for 48 hours whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 34, 38, 39 kDa
    Observed band size: 39-60 kDa
    why is the actual band size different from the predicted?


    Exposure time: 48 seconds


    The actual band size higher than predicted is mainly due to glycosylation. The molecular weight observed is consistent with what has been described in the literature (PMID:11454312 and PMID:12370356).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL1 antibody [EPR22492-64] (ab252938)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL1 antibody [EPR22492-64] (ab252938)

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling KIR2DL1 with ab252938 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in natural killer (NK) cells in human endometrium (PMID: 7749980) is observed.

    Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Flow Cytometry - Anti-KIR2DL1 antibody [EPR22492-64] (ab252938)
    Flow Cytometry - Anti-KIR2DL1 antibody [EPR22492-64] (ab252938)

    Flow cytometric analysis of human peripheral blood mononuclear cells (PBMC) labeling KIR2DL1 with ab252938 at 1/50 (right) compared with a Rabbit monoclonal IgG (ab172730) (left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150097), at 1/5000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG (Left) or ab252938 (Right). Then stained with anti-CD56 conjugated to BV421. Gated on viable cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL1 antibody [EPR22492-64] (ab252938)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL1 antibody [EPR22492-64] (ab252938)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling KIR2DL1 with ab252938 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in natural killer (NK) cells in human colon cancer (PMID: 7749980) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunoprecipitation - Anti-KIR2DL1 antibody [EPR22492-64] (ab252938)
    Immunoprecipitation - Anti-KIR2DL1 antibody [EPR22492-64] (ab252938)

    KIR2DL1 was immunoprecipitated from 0.35 mg NK-92 (Human malignant non-Hodgkin's lymphoma natural killer cell) treated with 2μM 5-aza-2-deoxycytidine for 48hrs, whole cell lysate with ab252938 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab252938 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

    Lane 1: NK-92 whole cell lysate (treated as above) 10 µg (Input).
    Lane 2: ab252938 IP in NK-92 whole cell lysate (treated as above).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252938 in NK-92 whole cell lysate (treated as above).

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 30 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL1 antibody [EPR22492-64] (ab252938)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL1 antibody [EPR22492-64] (ab252938)

    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling KIR2DL1 with ab252938 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in natural killer (NK) cells in human gastric cancer (PMID: 7749980) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1 antibody [EPR22492-64] (ab252938)
    Anti-KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1 antibody [EPR22492-64] (ab252938)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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