Antibodies, Proteins, Kits and Reagents for Life Science

Anti-SLAMF7/CS1 antibody [EPR21155] - BSA and Azide free (ab233090)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21155] to SLAMF7/CS1 - BSA and Azide free
Suitable for: WB, IHC-P, IP
Reacts with: Human

Product name

Anti-SLAMF7/CS1 antibody [EPR21155] - BSA and Azide free
See all SLAMF7/CS1 primary antibodies
Description

Rabbit monoclonal [EPR21155] to SLAMF7/CS1 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, IPmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human multiple myeloma tissue.
General notes
ab233090 is the carrier-free version of ab230945 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab233090 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.

This product was previously labelled as SLAMF7
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21155
Isotype

IgG
Research areas

Immunoprecipitation - Anti-SLAMF7/CS1 antibody [EPR21155] - BSA and Azide free (ab233090)

SLAMF7/CS1 was immunoprecipitated from 0.35 mg of IM-9 (human multiple myeloma B lymphoblast cell line) whole cell lysate with ab230945 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230945 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1: IM-9 whole cell lysate 10 μg (Input).

Lane 2: ab230945 IP in IM-9 whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230945 in IM-9 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 8 seconds.

The expression profile observed is consistent with what has been described in the literature (PMID: 18451245; 11698418; 25312647), with the bands greater than 37 kDa predicted to be glycosylated SLAMF7/CS1.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230945).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [EPR21155] - BSA and Azide free (ab233090)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling SLAMF7/CS1 with ab230945 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly cytoplasmic staining in plasma cells of human colon (PMID: 24299175) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230945).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [EPR21155] - BSA and Azide free (ab233090)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling SLAMF7/CS1 with ab230945 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly cytoplasmic staining in plasma cells of human tonsil is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230945).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [EPR21155] - BSA and Azide free (ab233090)

Immunohistochemical analysis of paraffin-embedded human multiple myeloma tissue labeling SLAMF7/CS1 with ab230945 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly cytoplasmic staining in human multiple myeloma (PMID: 26005365; PMID: 18451245) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230945).
Anti-SLAMF7/CS1 antibody [EPR21155] - BSA and Azide free (ab233090)

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