Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Wednesday March 10, 2021

Anti-SLAMF7/CS1 antibody [EPR21155] (ab230945)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21155] to SLAMF7/CS1
Suitable for: WB, IHC-P, IP
Reacts with: Human

Product name

Anti-SLAMF7/CS1 antibody [EPR21155]
See all SLAMF7/CS1 primary antibodies
Description

Rabbit monoclonal [EPR21155] to SLAMF7/CS1
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human tonsil lysate; IM-9 whole cell lysate. IHC-P: Human tonsil, colon and human multiple myeloma tissue. IP: IM-9 whole cell lysate.
General notes
This product was previously labelled as SLAMF7

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21155
Isotype

IgG
Research areas

Immunoprecipitation - Anti-SLAMF7/CS1 antibody [EPR21155] (ab230945)

SLAMF7/CS1 was immunoprecipitated from 0.35 mg of IM-9 (human multiple myeloma B lymphoblast cell line) whole cell lysate with ab230945 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230945 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1: IM-9 whole cell lysate 10 μg (Input).

Lane 2: ab230945 IP in IM-9 whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230945 in IM-9 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 8 seconds.

The expression profile observed is consistent with what has been described in the literature (PMID: 18451245; 11698418; 25312647), with the bands greater than 37 kDa predicted to be glycosylated SLAMF7/CS1.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [EPR21155] (ab230945)

Immunohistochemical analysis of paraffin-embedded human multiple myeloma tissue labeling SLAMF7/CS1 with ab230945 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly cytoplasmic staining in human multiple myeloma (PMID: 26005365; PMID: 18451245) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [EPR21155] (ab230945)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling SLAMF7/CS1 with ab230945 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly cytoplasmic staining in plasma cells of human colon (PMID: 24299175) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [EPR21155] (ab230945)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling SLAMF7/CS1 with ab230945 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly cytoplasmic staining in plasma cells of human tonsil is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-SLAMF7/CS1 antibody [EPR21155] (ab230945)

All lanes : Anti-SLAMF7/CS1 antibody [EPR21155] (ab230945) at 1/1000 dilution

Lane 1 : Human tonsil tissue lysate
Lane 2 : IM-9 (human multiple myeloma B lymphoblast cell line), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 37 kDa
Observed band size: 37,60 kDa why is the actual band size different from the predicted?

Blocking/diluting buffer and concentration: 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID: 18451245, 11698418, 25312647), with the bands greater than 37 kDa predicted to be glycosylated SLAMF7/CS1.
Anti-SLAMF7/CS1 antibody [EPR21155] (ab230945)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com