Porcine FGF19 ELISA Kit (ab273220)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 20.48 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Pig
Overview
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Product name
Porcine FGF19 ELISA Kit
See all FGF19 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
10 pg/ml -
Range
20.48 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 112.9 98% - 145% Serum 141.8 135% - 149% Plasma 139.2 127% - 146% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Pig -
Product overview
Porcine FGF19 ELISA Kit (ab273220) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine FGF-19 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for porcine FGF-19 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF-19 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-porcine FGF-19 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF-19 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Porcine FGF19 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Porcine FGF19 Antibody 2 vials Porcine FGF19 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB. -
Tissue specificity
Expressed in fetal brain, cartilage, retina, and adult gall bladder. -
Sequence similarities
Belongs to the heparin-binding growth factors family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- FGF 19
- FGF-19
- FGF15
see all
Images
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Porcine FGF-19 Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Porcine FGF-19 ELISA kit (ab273220) Standard curve.