Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD94 antibody [EPR21003] (ab235441)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21003] to CD94
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-CD94 antibody [EPR21003]
    See all CD94 primary antibodies

  • Description

    Rabbit monoclonal [EPR21003] to CD94

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NK-92 whole cell lysate. IHC-P: Human tonsil and NK/T-cell lymphoma tissue. IP: NK-92 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR21003

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD94 antibody [EPR21003] (ab235441)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD94 antibody [EPR21003] (ab235441)

    Immunohistochemical analysis of paraffin-embedded human NK/T-cell lymphoma tissue labeling CD94 with ab235441 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic staining in human NK/T-cell lymphoma (PMID: 11253136, PMID: 10828054, PMID: 12816864) is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-CD94 antibody [EPR21003] (ab235441)
    Western blot - Anti-CD94 antibody [EPR21003] (ab235441)
    Anti-CD94 antibody [EPR21003] (ab235441) at 1/1000 dilution + NK-92 (Human peripheral blood malignant non-Hodgkin's lymphoma cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 20 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 10358137; PMID: 8969479). The diffuse bands between 20-30 kDa are likely due to glycosylation.

     

  • Immunoprecipitation - Anti-CD94 antibody [EPR21003] (ab235441)
    Immunoprecipitation - Anti-CD94 antibody [EPR21003] (ab235441)

    CD94 was immunoprecipitated from 0.35 mg NK-92 (Human peripheral blood malignant non-Hodgkin's lymphoma cell line) whole cell lysate with ab235441 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab235441 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
    Lane 1: NK-92 whole cell lysate 10 µg (Input).
    Lane 2: ab235441 IP in NK-92 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab235441 in NK-92 whole cell lysate (-).
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 10 seconds.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 10358137; PMID: 8969479). The diffuse bands between 20-30 kDa are likely due to glycosylation.

    This blot was developed with a high sensitivity ECL substrate.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD94 antibody [EPR21003] (ab235441)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD94 antibody [EPR21003] (ab235441)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD94 with ab235441 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in natural killer (NK) cells in the marginal zone of human tonsil (PMID: 11253136) is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-CD94 antibody [EPR21003] (ab235441)
    Anti-CD94 antibody [EPR21003] (ab235441)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CD94 antibody [EPR21003] (ab235441)

© 2021 Astana Biomed Group LLP. All rights reserved.