Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

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Anti-CD94 antibody [EPR21003] (ab235441)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21003] to CD94
Suitable for: WB, IHC-P, IP
Reacts with: Human

Product name

Anti-CD94 antibody [EPR21003]
See all CD94 primary antibodies
Description

Rabbit monoclonal [EPR21003] to CD94
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: NK-92 whole cell lysate. IHC-P: Human tonsil and NK/T-cell lymphoma tissue. IP: NK-92 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21003
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD94 antibody [EPR21003] (ab235441)

Immunohistochemical analysis of paraffin-embedded human NK/T-cell lymphoma tissue labeling CD94 with ab235441 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic staining in human NK/T-cell lymphoma (PMID: 11253136, PMID: 10828054, PMID: 12816864) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-CD94 antibody [EPR21003] (ab235441)

Anti-CD94 antibody [EPR21003] (ab235441) at 1/1000 dilution + NK-92 (Human peripheral blood malignant non-Hodgkin's lymphoma cell line) whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 20 kDa

Exposure time: 15 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular mass observed is consistent with what has been described in the literature (PMID: 10358137; PMID: 8969479). The diffuse bands between 20-30 kDa are likely due to glycosylation.
Immunoprecipitation - Anti-CD94 antibody [EPR21003] (ab235441)

CD94 was immunoprecipitated from 0.35 mg NK-92 (Human peripheral blood malignant non-Hodgkin's lymphoma cell line) whole cell lysate with ab235441 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab235441 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: NK-92 whole cell lysate 10 µg (Input).
Lane 2: ab235441 IP in NK-92 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab235441 in NK-92 whole cell lysate (-).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.

The molecular mass observed is consistent with what has been described in the literature (PMID: 10358137; PMID: 8969479). The diffuse bands between 20-30 kDa are likely due to glycosylation.

This blot was developed with a high sensitivity ECL substrate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD94 antibody [EPR21003] (ab235441)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD94 with ab235441 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in natural killer (NK) cells in the marginal zone of human tonsil (PMID: 11253136) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-CD94 antibody [EPR21003] (ab235441)

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