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Mouse/Rat Cytochrome C ELISA Kit (ab210575)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 44 pg/ml
  • Range: 487.4 pg/ml - 14000 pg/ml
  • Sample type: Cell culture extracts, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Mouse/Rat Cytochrome C ELISA Kit
    See all Cytochrome C kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    NIH3T3 ext 5 2.7%
    Inter-assay
    Sample n Mean SD CV%
    NIH3T3 ext 3 5.3%

  • Sample type

    Cell culture extracts, Tissue Extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    44 pg/ml

  • Range

    487.4 pg/ml - 14000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Tissue Extracts 108 106% - 112%
    Cell culture media 111 106% - 116%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse, Rat

  • Product overview

    Mouse/Rat Cytochrome C ELISA Kit (ab210575) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cytochrome C protein in tissue extracts, cell culture media, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse/Rat Cytochrome C with 44 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Cytochrome C is 11 kDa mitochondrial intermembrane space electron carrier protein. The oxidized form of the cytochrome C heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome C then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.

    Cytochrome C also plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial outer membrane permeability resulting in release of cytochrome C into the cytosol. Binding of cytochrome C to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse/Rat Cytochrome C ELISA Kit (ab210575)
    Other - Mouse/Rat Cytochrome C ELISA Kit (ab210575)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Mouse/Rat Cytochrome C standard curve comparison data.
    Mouse/Rat Cytochrome C standard curve comparison data.

    Standard curve comparison between mouse/rat Cytochrome C SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 10-fold increase in sensitivity.

  • Example of mouse/rat Cytochrome C standard curve.
    Example of mouse/rat Cytochrome C standard curve.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native Cytochrome C in mouse heart tissue extract samples based on 15 µg/mL extract load and in rat heart tissue extract samples based on 7 µg/mL extract load.
    Interpolated concentrations of native Cytochrome C in mouse heart tissue extract samples based on 15 µg/mL extract load and in rat heart tissue extract samples based on 7 µg/mL extract load.

    The concentrations of Cytochrome C were measured in duplicate and interpolated from the Cytochrome C standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytochrome C concentration was determined to be 8,822 pg/mL in mouse heart extract and 4,109 pg/mL in rat heart extract samples.

  • Interpolated concentrations of native Cytochrome C in mouse NIH/3T3 cell extract samples based on 65 µg/mL extract load, and mouse C2C12 and rat C6 cell extract samples based on 100 µg/mL extract.
    Interpolated concentrations of native Cytochrome C in mouse NIH/3T3 cell extract samples based on 65 µg/mL extract load, and mouse C2C12 and rat C6 cell extract samples based on 100 µg/mL extract.

    The concentrations of Cytochrome C were measured in duplicate and interpolated from the Cytochrome C standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytochrome C concentration was determined to be 10,516 pg/mL in NIH/3T3 extract, 11,096 pg/mL in C2C12 extract, and 13,297 pg/mL in C6 extract.

  • Interpolated concentrations of native Cytochrome C in mouse and rat extract samples.
    Interpolated concentrations of native Cytochrome C in mouse and rat extract samples.

    The concentrations of Cytochrome C were measured in three different dilutions in duplicate and interpolated from the Cytochrome C standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng of Cytochrome C per mg of extract (mean +/-SD, n=3). Cytochrome C concentration was determined to be 601 ng/mg in mouse heart, 622 ng/mg in rat heart, 166 ng/mg in mouse NIH/3T3 cell, 105 ng/mg in mouse C2C12 cell and 136 ng/mg in rat C6 cell extract samples.

  • Sandwich ELISA - Mouse/Rat Cytochrome C ELISA Kit (ab210575)
    Sandwich ELISA - Mouse/Rat Cytochrome C ELISA Kit (ab210575)
    To learn more about the advantages of recombinant antibodies see here.

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