Human Furin ELISA Kit (ab113322)
Key features and details
- Sensitivity: 15 pg/ml
- Range: 13.72 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Furin ELISA Kit -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
Range
13.72 pg/ml - 10000 pg/mlRecovery
84 %
Sample specific recovery Sample type Average % Range Serum 89.57 80% - 98% Plasma 77.74 69% - 85% Cell culture media 84.69 75% - 93% Assay duration
Multiple steps standard assaySpecies reactivity
Reacts with: HumanProduct overview
Abcam’s Furin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Furin in serum, plasma (collect plasma using heparin and EDTA as an anticoagulant. Citrate is not recommended) and cell culture supernatants.
This assay employs an antibody specific for Human Furin coated on a 96-well plate. Standards and samples are pipetted into the wells and Furin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Furin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Furin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Notes
Optimization may be required with urine samples.
Platform
MicroplateProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human Furin (lyophilized) Item F 2 vials Furin Microplate (12 x 8 wells) Item A 1 unit Recombinant Human Furin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Database links
- Entrez Gene: 5045 Human
- SwissProt: P09958 Human
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human Furin (lyophilized) Item F 2 vials Furin Microplate (12 x 8 wells) Item A 1 unit Recombinant Human Furin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Database links
- Entrez Gene: 5045 Human
- SwissProt: P09958 Human
Images
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Typical data B
Representative standard curve using ab113322
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Typical data ARepresentative standard curve using ab113322