Anti-Peroxiredoxin Asp f3 antibody (ab225925)
Key features and details
- Rabbit polyclonal to Peroxiredoxin Asp f3
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Peroxiredoxin Asp f3 antibody -
Description
Rabbit polyclonal to Peroxiredoxin Asp f3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant full length protein corresponding to Peroxiredoxin Asp f3 aa 1 to the C-terminus. Neosartorya fumigata. Immunogen has 6 His-SUMO tag at N-terminus.
Database link: O43099 -
Positive control
- WB: Recombinant protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG
Images
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Western blot - Anti-Peroxiredoxin Asp f3 antibody (ab225925)All lanes : Anti-Peroxiredoxin Asp f3 antibody (ab225925) at 1/500 dilution
Lane 1 : Recombinant protein 100 ng
Lane 2 : Recombinant protein 50 ng
Lane 3 : Recombinant protein 25 ng
Lane 4 : Recombinant protein 12.5 ng
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Developed using the ECL technique.
Observed band size: 35 kDa why is the actual band size different from the predicted?The recombinant protein used in this western blot is tagged with 6 His-SUMO tag at N-terminus.
Expected molecular weight of tag: 16kDa
Expected molecular weight of Peroxiredoxin Asp f3: 19kDa