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Immunology Adaptive Immunity T Cells Non-CD

Anti-NFAT1 (phospho S54) antibody (ab200819)

Price and availability

278 083 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-NFAT1 (phospho S54) antibody (ab200819)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to NFAT1 (phospho S54)
  • Suitable for: WB
  • Reacts with: Mouse
  • Isotype: IgG

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Overview

  • Product name

    Anti-NFAT1 (phospho S54) antibody
    See all NFAT1 primary antibodies
  • Description

    Rabbit polyclonal to NFAT1 (phospho S54)
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Mouse NFAT1 (phospho S54).
    Database link: Q60591

  • Positive control

    • Murine T-cells + PMA + Ionomycin.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

    PBS without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab200819 was negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated NFAT1. The final product is generated by affinity chromatography using a NFAT1-derived peptide phosphorylated at serine 54.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Non-CD
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFATS
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFATs
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Transcription factors
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Images

  • Western blot - Anti-NFAT1 (phospho S54) antibody (ab200819)
    Western blot - Anti-NFAT1 (phospho S54) antibody (ab200819)
    All lanes : Anti-NFAT1 (phospho S54) antibody (ab200819) at 1/1000 dilution

    Lane 1 : Extracts of Murine T cells were left untreated with no peptide
    Lane 2 : Extracts of Murine T cells were treated with PMA and Ca2+ ionophore
    ionomycin with no peptide
    Lane 3 : Extracts of Murine T cells were treated with PMA and Ca2+ ionophore
    ionomycin with the non-phosphopeptide corresponding to the phosphopeptide immunogen
    Lane 4 : Extracts of Murine T cells were treated with PMA and Ca2+ ionophore
    ionomycin with a generic phosphoserine-containing peptide
    Lane 5 : Extracts of Murine T cells were treated with PMA and Ca2+ ionophore
    ionomycin with the phosphopeptide immunogen

    Secondary
    All lanes : Goat F(ab’)2 anti-rabbit IgG alkaline phosphatase

    Predicted band size: 100 kDa



    Resolved by SDS-PAGE on a 10% Tris-glycine gel

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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