Anti-NFAT1 (phospho S54) antibody (ab200819)
Key features and details
- Rabbit polyclonal to NFAT1 (phospho S54)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-NFAT1 (phospho S54) antibody
See all NFAT1 primary antibodies -
Description
Rabbit polyclonal to NFAT1 (phospho S54) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse -
Immunogen
Synthetic peptide corresponding to Mouse NFAT1 (phospho S54).
Database link: Q60591 -
Positive control
- Murine T-cells + PMA + Ionomycin.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab200819 was negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated NFAT1. The final product is generated by affinity chromatography using a NFAT1-derived peptide phosphorylated at serine 54. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-NFAT1 (phospho S54) antibody (ab200819) at 1/1000 dilution
Lane 1 : Extracts of Murine T cells were left untreated with no peptide
Lane 2 : Extracts of Murine T cells were treated with PMA and Ca2+ ionophore
ionomycin with no peptide
Lane 3 : Extracts of Murine T cells were treated with PMA and Ca2+ ionophore
ionomycin with the non-phosphopeptide corresponding to the phosphopeptide immunogen
Lane 4 : Extracts of Murine T cells were treated with PMA and Ca2+ ionophore
ionomycin with a generic phosphoserine-containing peptide
Lane 5 : Extracts of Murine T cells were treated with PMA and Ca2+ ionophore
ionomycin with the phosphopeptide immunogen
Secondary
All lanes : Goat F(ab’)2 anti-rabbit IgG alkaline phosphatase
Predicted band size: 100 kDaResolved by SDS-PAGE on a 10% Tris-glycine gel