Anti-Orai1 antibody (ab244352)
Key features and details
- Rabbit polyclonal to Orai1
- Suitable for: WB, ICC, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Orai1 antibody
See all Orai1 primary antibodies -
Description
Rabbit polyclonal to Orai1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment within Human Orai1 aa 250 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q96D31 -
Positive control
- IHC-P: Human skin and duodenum tissues; WB: NB4 whole cell lysate; ICC: U-251 MG cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Formalin-fixed, paraffin-embedded human skin tissue stained for Orai1 with ab244352 at a 1/50 dilution in immunohistochemical analysis.
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PFA fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells labeling Orai1 using ab244352 at 4 µg/ml (green) in ICC.
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Anti-Orai1 antibody (ab244352) at 0.4 µg/ml + NB4 (human cell line) lysate
Predicted band size: 33 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human duodenum tissue labelling Orai1 with ab244352 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue labelling Orai1 with ab244352 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling Orai1 with ab244352 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.