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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Methylation

Anti-KDM2A antibody (ab174693)

Anti-KDM2A antibody (ab174693)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Goat polyclonal to KDM2A
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-KDM2A antibody
    See all KDM2A primary antibodies
  • Description

    Goat polyclonal to KDM2A
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Hamster, Dog, Pig, Monkey
  • Immunogen

    Synthetic peptide corresponding to Human KDM2A aa 445-459 (internal sequence) (Cysteine residue).
    Sequence:

    PRKDRQVHLTHFELE


    Database link: NP_036440.1
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human small intestine tissue; Human uterus lysate.
  • General notes

     This product was previously labelled as FBXL11

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Jumonji containing proteins
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Lysine demethylation

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM2A antibody (ab174693)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM2A antibody (ab174693)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue labeling KDM2A with ab174693 at 5 µg/ml.

  • Western blot - Anti-KDM2A antibody (ab174693)
    Western blot - Anti-KDM2A antibody (ab174693)
    Anti-KDM2A antibody (ab174693) at 0.5 µg/ml + Human uterus lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 133 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Anti-KDM2A antibody (ab240747)

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    Anti-KDM2A antibody [EPR18602] (ab191387)

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