Anti-KDM2A antibody (ab240747)
Key features and details
- Rabbit polyclonal to KDM2A
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-KDM2A antibody
See all KDM2A primary antibodies -
Description
Rabbit polyclonal to KDM2A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman
-
Immunogen
Synthetic peptide within Human KDM2A aa 1112-1162. The exact sequence is proprietary. (NP_036440.1 )
Database link: Q9Y2K7 -
Positive control
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: KDM2A IP in HeLa whole cell lysate.
-
General notes
This product was previously labelled as FBXL11
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-KDM2A antibody (ab240747)All lanes : Anti-KDM2A antibody (ab240747) at 0.2 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 133 kDa
Exposure time: 3 minutesLysates prepared using NETN lysis buffer.
-
Immunoprecipitation - Anti-KDM2A antibody (ab240747)KDM2A was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab240747 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab240747 at 1 µg/ml.
Lane 1: ab240747 (batch 1) IP in HeLa whole cell lysate.
Lane 2: ab240747 (batch 2) IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
Prepared using NETN lysis buffer.
