Human ULBP3 ELISA Kit (ab267617)
Key features and details
- Sensitivity: 1.3 pg/ml
- Range: 1.229 pg/ml - 300 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ULBP3 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.3 pg/ml -
Range
1.229 pg/ml - 300 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 81.54 74% - 92% Plasma 77.77 72% - 84% Cell culture media 77.14 69% - 80% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human ULBP3 ELISA Kit is designed for the quantitative determination of ULBP3 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human ULBP3 coated on a 96-well plate. Standards and samples are pipetted into the wells and ULBP3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human ULBP3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ULBP3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested AMIGO, Aminopeptidase LRAP (ERAP2), Amnionless, Arylsulfatase A, Bcl-w, CD109, CD157, CD34, CD83, CLEC1, CLEC10A (CD301), CMG-2, CREG, Cystatin SN, Cytokeratin-8, Dectin-1 (CLEC7A), Desmocollin-3, Endoglycan, Galectin-4, HAPLN1, Jagged 1, Langerin (CD207), Lumican, Matriptase (ST14), MEP1B, Nectin-3, OX40 (TNFRSF4), OX40 Ligand (TNFSF4), p27, Pappalysin2, Plexin B3, Plexin D1, proGRP, PSA-total, Reg1B, RGM-A, ROBO2, Spinesin, TWEAK R (TNFRSF12).
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Anti-Human ULBP3 coated Microplate (12 x 8 wells) 1 unit Assay Diluent C 1 x 30ml Biotinylated Anti-Human ULBP3 Detection Antibody 2 vials Human ULBP3 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Relevance
ULBP3 is a human ligand for the NKG2D receptor, together with at least ULBP1 and ULBP2. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. ULBP3 has a lower affinity for NKG2D compared to ULBP1 and ULBP2 and induces weaker signaling responses than does ULBP2 or ULBP1. -
Cellular localization
Cell Membrane -
Alternative names
- ALCAN- gamma
- N2DL-3
- N2DL3
see all -
Database links
- Entrez Gene: 79465 Human
- Omim: 605699 Human
- SwissProt: Q9BZM4 Human
- Unigene: 326556 Human
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.