Human PCSK9 ELISA Kit (ab209884)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 68 pg/ml
- Range: 0.5 ng/ml - 32 ng/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human PCSK9 ELISA Kit
See all PCSK9 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Human serum 5 4.4% Inter-assay Sample n Mean SD CV% Human serum 3 4.6% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
68 pg/ml -
Range
0.5 ng/ml - 32 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 106 103% - 109% Serum 105 104% - 107% Cell culture extracts 92 91% - 94% Hep Plasma 111 107% - 115% EDTA Plasma 96 93% - 100% Cit plasma 104 102% - 106% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow, Cynomolgus monkey, Macaque monkey -
Product overview
Human PCSK9 ELISA Kit (ab209884) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PCSK9 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PCSK9 with 68 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a crucial player in the regulation of plasma cholesterol homeostasis. Degradation of low-density lipid receptor family members (LDLR, VLDLR, APOER, and APOER2) is promoted in intracellular acidic compartments following binding by PCSK9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human PCSK9 Capture Antibody 1 x 600µl 10X Human PCSK9 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human PCSK9 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis. -
Tissue specificity
Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. -
Involvement in disease
Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant. -
Sequence similarities
Belongs to the peptidase S8 family.
Contains 1 peptidase S8 domain. -
Post-translational
modificationsThe soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Convertase subtilisin/kexin type 9 preproprotein
- FH3
- HCHOLA3
see all -
Database links
- Entrez Gene: 255738 Human
- Omim: 607786 Human
- SwissProt: Q8NBP7 Human
- Unigene: 18844 Human
Images
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Other - Human PCSK9 ELISA Kit (ab209884)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human PCSK9 standard curve comparison data.Standard curve comparison between human PCSK9 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 2-fold increase in sensitivity.
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Example of human PCSK9 standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Example of human PCSK9 standard curve in 1X Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
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. Interpolated concentrations of native PCSK9 in human serum and plasma samplesThe concentrations of PCSK9 were measured in duplicates, interpolated from the PCSK9 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PCSK9 concentration was determined to be 207 ng/mL in serum, 117 ng/mL in plasma (citrate), 166 ng/mL in plasma (EDTA), and 292 ng/mL in plasma (heparin).
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Interpolated concentrations of native PCSK9 in HepG2 cell culture supernatant and cell extractThe concentrations of PCSK9 were measured in duplicate, interpolated from the PCSK9 standard curve, and corrected for sample dilution. Undiluted samples are as follows: HepG2 cell culture supernatant 50%, HepG2 cell extract 500 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PCSK9 concentration was determined to be 7.3 ng/mL in HepG2 cell culture supernatant and 15 ng/mL in HepG2 cell extract.
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Serum from six individual human male donors was measured in duplicateInterpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PCSK9 concentration was determined to be 208 ng/mL with a range of 125 - 317 ng/mL.
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Sandwich ELISA - Human PCSK9 ELISA Kit (ab209884)To learn more about the advantages of recombinant antibodies see here.
