Human PCSK9 Matched Antibody Pair Kit (ab215081)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 250 pg/ml - 16000 pg/ml
Overview
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Product name
Human PCSK9 Matched Antibody Pair Kit
See all PCSK9 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
37.07 pg/ml -
Range
250 pg/ml - 16000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human PCSK9 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human PCSK9.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab209884. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: IA, ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human PCSK9 Capture Antibody 2 x 50µg 1 x 50µg Human PCSK9 Detector Antibody 2 x 12.5µg 1 x 12.5µg Human PCSK9 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis. -
Tissue specificity
Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. -
Involvement in disease
Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant. -
Sequence similarities
Belongs to the peptidase S8 family.
Contains 1 peptidase S8 domain. -
Post-translational
modificationsThe soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Convertase subtilisin/kexin type 9 preproprotein
- FH3
- HCHOLA3
see all -
Database links
- Entrez Gene: 255738 Human
- Omim: 607786 Human
- SwissProt: Q8NBP7 Human
- Unigene: 18844 Human
Images
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Human PCSK9 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human PCSK9 Matched Antibody Pair Kit (ab215081)To learn more about the advantages of recombinant antibodies see here.
