Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human PCSK9 ELISA Kit, Fluorescent (ab229406)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 37 pg/ml
  • Range: 0.06 ng/ml - 32 ng/ml
  • Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
  • Detection method: Fluorescent
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human PCSK9 ELISA Kit, Fluorescent
    See all PCSK9 kits

  • Detection method

    Fluorescent

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 4.4%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 4.6%

  • Sample type

    Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    37 pg/ml

  • Range

    0.06 ng/ml - 32 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 106 103% - 109%
    Serum 105 104% - 107%
    Cell culture extracts 92 91% - 94%
    Hep Plasma 111 107% - 115%
    EDTA Plasma 96 93% - 100%
    Cit plasma 104 102% - 106%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human
    Does not react with: Cow

  • Product overview

    PCSK9 in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PCSK9 protein in human serum, plasmas, cell culture supernatant, and cell extracts.


    This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
    If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.


    The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.

  • Notes

    Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a crucial player in the regulation of plasma cholesterol homeostasis. Degradation of low-density lipid receptor family members (LDLR, VLDLR, APOER, and APOER2) is promoted in intracellular acidic compartments following binding by PCSK9.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Human PCSK9 ELISA Kit, Fluorescent (ab229406)
    Other - Human PCSK9 ELISA Kit, Fluorescent (ab229406)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of human PCSK9 standard curve in Sample Diluent NS
    Example of human PCSK9 standard curve in Sample Diluent NS

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native PCSK9 in human serum and plasma samples
    Interpolated concentrations of native PCSK9 in human serum and plasma samples

    The concentrations of PCSK9 were measured in duplicates, interpolated from the PCSK9 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PCSK9 concentration was determined to be 207 ng/mL in serum, 117 ng/mL in plasma (citrate), 166 ng/mL in plasma (EDTA), and 292 ng/mL in plasma (heparin).

  • Interpolated concentrations of native PCSK9 in HepG2 cell culture supernatant and cell extract
    Interpolated concentrations of native PCSK9 in HepG2 cell culture supernatant and cell extract

    The concentrations of PCSK9 were measured in duplicate, interpolated from the PCSK9 standard curve, and corrected for sample dilution. Undiluted samples are as follows: HepG2 cell culture supernatant 50%, HepG2 cell extract 500 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PCSK9 concentration was determined to be 7.3 ng/mL in HepG2 cell culture supernatant and 15 ng/mL in HepG2 cell extract.

  • Serum from six individual human male donors was measured in duplicate
    Serum from six individual human male donors was measured in duplicate

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PCSK9 concentration was determined to be 208 ng/mL with a range of 125 - 317 ng/mL.

  • Sandwich ELISA - Human PCSK9 ELISA Kit, Fluorescent (ab229406)
    Sandwich ELISA - Human PCSK9 ELISA Kit, Fluorescent (ab229406)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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