Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Liver Arginase antibody [EPR22033-369] - BSA and Azide free (ab259271)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22033-369] to Liver Arginase - BSA and Azide free
  • Suitable for: IHC-P, IP, WB, Flow Cyt
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Liver Arginase antibody [EPR22033-369] - BSA and Azide free
    See all Liver Arginase primary antibodies

  • Description

    Rabbit monoclonal [EPR22033-369] to Liver Arginase - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, WB, Flow Cytmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse liver, Mouse kidney, Rat liver and Rat kidney lysates. IHC-P: Mouse liver and Rat liver tissues. Flow Cyt: Hepa1-6 cells. IP: Mouse liver tissue lysate

  • General notes

    Ab259271 is the carrier-free version of ab233548. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab259271 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [EPR22033-369] - BSA and Azide free (ab259271)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [EPR22033-369] - BSA and Azide free (ab259271)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Liver Arginase with ab233548 at 1/2000 dilution (0.333 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear and cytoplasmic staining in rat liver (PMID/ 22298472, 23637937, 21975054) is observed. The section was incubated with ab233548 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233548).

  • Western blot - Anti-Liver Arginase antibody [EPR22033-369] - BSA and Azide free (ab259271)
    Western blot - Anti-Liver Arginase antibody [EPR22033-369] - BSA and Azide free (ab259271)
    All lanes : Anti-Liver Arginase antibody [EPR22033-369] (ab233548) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 35 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed in RAW 264.7 is consistent with the literature (PMID: 28032600, PMID: 27166374, PMID: 9814991, PMID: 11080091).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233548).

  • Flow Cytometry - Anti-Liver Arginase antibody [EPR22033-369] - BSA and Azide free (ab259271)
    Flow Cytometry - Anti-Liver Arginase antibody [EPR22033-369] - BSA and Azide free (ab259271)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Hepa1-6 (mouse epithelial hepatoma) cells labeling Liver Arginase with ab233548 at 1/60 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233548).

  • Immunoprecipitation - Anti-Liver Arginase antibody [EPR22033-369] - BSA and Azide free (ab259271)
    Immunoprecipitation - Anti-Liver Arginase antibody [EPR22033-369] - BSA and Azide free (ab259271)

    Liver Arginase was immunoprecipitated from 0.35 mg Mouse liver tissue lysate with ab233548 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab233548 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse liver tissue lysate 10ug

    Lane 2: ab233548 IP in Mouse liver tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab233548 in Mouse liver tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    Exposure time: 3 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233548).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [EPR22033-369] - BSA and Azide free (ab259271)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [EPR22033-369] - BSA and Azide free (ab259271)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Liver Arginase with ab233548 at 1/2000 dilution (0.333 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear and cytoplasmic staining in mouse liver (PMID/ 22298472, 23637937, 21975054). The section was incubated with ab233548 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233548).

  • Anti-Liver Arginase antibody [EPR22033-369] - BSA and Azide free (ab259271)
    Anti-Liver Arginase antibody [EPR22033-369] - BSA and Azide free (ab259271)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Liver Arginase antibody [EPR22033-369] - BSA and Azide free (ab259271)

© 2021 Astana Biomed Group LLP. All rights reserved.