Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸ 10 µl 137 366 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Liver Arginase antibody [EPR6672(B)] (ab133543)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6672(B)] to Liver Arginase
Suitable for: WB, IHC-P, IP
Reacts with: Human

Product name

Anti-Liver Arginase antibody [EPR6672(B)]
See all Liver Arginase primary antibodies
Description

Rabbit monoclonal [EPR6672(B)] to Liver Arginase
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Liver Arginase aa 300 to the C-terminus. The exact sequence is proprietary.
Database link: P05089
(Peptide available as ab217538)
Positive control
- WB: Human fetal liver and fetal lung lysates. IHC-P: Human hepatocellular carcinoma tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6672(B)
Isotype

IgG
Research areas

Western blot - Anti-Liver Arginase antibody [EPR6672(B)] (ab133543)

Anti-Liver Arginase antibody [EPR6672(B)] (ab133543) at 1/2000 dilution + Human liver lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 35 kDa

Exposure time: 5 seconds

Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [EPR6672(B)] (ab133543)

ab133543 staining liver arginase in paraffin embedded human hepatocellular cancer tissue sections by Immunohistochemistry.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

Samples were incubated with primary antibody at 1:2000 dilution (0.13 μg/ml).

A ready to use Goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody.

Hematoxylin was used as a counterstain.

Cytoplasmic and nuclear staining on human hepatocellular cancer.
Immunoprecipitation - Anti-Liver Arginase antibody [EPR6672(B)] (ab133543)

Lane 1 (input): Human fetal liver whole cell lysate, 10μg
Lane 2: Human fetal liver whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab133543 in Human fetal liver whole cell lysate

Ab133543 immunoprecipitating liver arginase in Human fetal liver whole cell lysates. Primary antibody was used at a 1:1000 dilution (0.27 μg/ml). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:5000 dilution. Capture antibody was used at 1:20 dilution (1.3μg in 0.35mg lysates).

Blocking and diluting buffer used: 5% NFDM/TBST.
Anti-Liver Arginase antibody [EPR6672(B)] (ab133543)

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