Anti-SHMT2/SHMT antibody (ab233430)
Key features and details
- Rabbit polyclonal to SHMT2/SHMT
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-SHMT2/SHMT antibody
See all SHMT2/SHMT primary antibodies -
Description
Rabbit polyclonal to SHMT2/SHMT -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Rabbit, Cow
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Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Human SHMT2/SHMT aa 1 to the C-terminus. Mature chain. Two N-terminal tags. Expressed in E.coli.
Database link: P34897 -
Positive control
- WB: Recombinant human SHMT2/SHMT protein; Human lung lysate; HEK-293T and HeLa cell lysates; Pig pancreas lysate. IHC-P: Human breast cancer, stomach, stomach cancer, rectum cancer, breast cancer, prostate and cerebrum tissue. ICC/IF: HeLa cells.
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General notes
This product was previously labelled as SHMT2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SHMT2/SHMT antibody (ab233430)Anti-SHMT2/SHMT antibody (ab233430) at 1 µg/ml + Recombinant human SHMT2/SHMT protein
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 56 kDa
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Western blot - Anti-SHMT2/SHMT antibody (ab233430)All lanes : Anti-SHMT2/SHMT antibody (ab233430) at 1 µg/ml
Lane 1 : Human lung lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 4 : Pig pancreas lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 56 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (ab233430)Formalin-fixed, paraffin-embedded human breast cancer tissue stained for SHNT2 using ab233430 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (ab233430)Formalin-fixed, paraffin-embedded human stomach tissue stained for SHNT2 using ab233430 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (ab233430)Formalin-fixed, paraffin-embedded human stomach cancer tissue stained for SHNT2 using ab233430 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (ab233430)Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for SHNT2 using ab233430 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (ab233430)Formalin-fixed, paraffin-embedded human prostate tissue stained for SHNT2 using ab233430 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (ab233430)Formalin-fixed, paraffin-embedded human cerebrum tissue stained for SHNT2 using ab233430 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody (ab233430)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SHMT2/SHMT (green) using ab233430 at 20 μg/ml in ICC/IF. FITC staining.
