Anti-SHMT2/SHMT antibody (ab224428)
Key features and details
- Rabbit polyclonal to SHMT2/SHMT
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SHMT2/SHMT antibody
See all SHMT2/SHMT primary antibodies -
Description
Rabbit polyclonal to SHMT2/SHMT -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow
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Immunogen
Recombinant fragment corresponding to Human SHMT2/SHMT aa 400-500.
Database link: P34897 -
Positive control
- WB: RT4, RH-30, U-251 MG, NIH/3T3 and NBT-II cell lysates. IHC-P: Human lymph node, skin, colon, liver and kidney tissue ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (ab224428)
Paraffin-embedded human skin tissue stained for SHMT2/SHMT using ab224428 at 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (ab224428)Paraffin-embedded human kidney tissue stained for SHMT2/SHMT using ab224428 at 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (ab224428)Paraffin-embedded human colon tissue stained for SHMT2/SHMT using ab224428 at 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (ab224428)Paraffin-embedded human liver tissue stained for SHMT2/SHMT using ab224428 at 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (ab224428)Paraffin-embedded human skeletal muscle tissue stained for SHMT2/SHMT using ab224428 at 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (ab224428)Paraffin-embedded human stomach tissue stained for SHMT2/SHMT using ab224428 at 1/200 dilution in immunohistochemical analysis.
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Western blot - Anti-SHMT2/SHMT antibody (ab224428)All lanes : Anti-SHMT2/SHMT antibody (ab224428) at 1/100 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Predicted band size: 56 kDa
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Western blot - Anti-SHMT2/SHMT antibody (ab224428)Anti-SHMT2/SHMT antibody (ab224428) at 100 µg + RH-30 cell lysate
Predicted band size: 56 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (ab224428)Paraffin-embedded human lymph node tissue stained for SHMT2/SHMT using ab224428 at 1/200 dilution in immunohistochemical analysis.
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Western blot - Anti-SHMT2/SHMT antibody (ab224428)All lanes : Anti-SHMT2/SHMT antibody (ab224428) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate
Lane 2 : NBT-II cell lysate
Developed using the ECL technique.
Predicted band size: 56 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody (ab224428)PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for SHMT2/SHMT (green) using ab224428 at 4 µg/ml in ICC/IF.
