Anti-TGF beta Receptor I antibody (ab121024)
Key features and details
- Goat polyclonal to TGF beta Receptor I
- Suitable for: IHC-P, Flow Cyt (Intra), WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TGF beta Receptor I antibody
See all TGF beta Receptor I primary antibodies -
Description
Goat polyclonal to TGF beta Receptor I -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Rat
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Immunogen
Synthetic peptide within Human TGF beta Receptor I aa 50-150 (internal sequence). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Corresponds to NP_004603.1 and NP_001124388.1.
Database link: P36897 -
Positive control
- WB: Rat liver and lung tissue lysates. IHC-P: Human adrenal gland and small intestine tissues. Flow Cyt (Intra): HeLa cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab121024 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TGF beta Receptor I antibody (ab121024)Lane 1 : Anti-TGF beta Receptor I antibody (ab121024) at 0.1 µg/ml
Lane 2 : Anti-TGF beta Receptor I antibody (ab121024) at 0.03 µg/ml
Lane 1 : Rat liver lysate in RIPA buffer
Lane 2 : Rat lung lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 55 kDa
Observed band size: 55 kDaPrimary incubation 1 hour at room temperature. Detected by chemiluminescence.
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Flow Cytometry (Intracellular) - Anti-TGF beta Receptor I antibody (ab121024)Flow cytometric (Intracellular) analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
