Anti-TGF beta Receptor I antibody (ab31013)
Key features and details
- Rabbit polyclonal to TGF beta Receptor I
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TGF beta Receptor I antibody
See all TGF beta Receptor I primary antibodies -
Description
Rabbit polyclonal to TGF beta Receptor I -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human TGF beta Receptor I aa 150-250. The exact sequence is proprietary.
Database link: P36897 -
Positive control
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TGF beta Receptor I antibody (ab31013) Verified customerAll lanes :
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate withAnti-TGF beta Receptor I antibody (ab31013)
Lane 2 : A2780 (Human ovarian cancer cell line) whole cell lysate withAnti-TGF beta Receptor I antibody (ab31013)
Lysates/proteins at 20 µg per lane.
Blocking peptides at 1/1000 dilution per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Exposure time: 15 secondsPrimary antibody incubated for 15 hours at 4°C in TBST.
3% BSA used as blocking agent for 1 hour at 23°C.
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Western blot - Anti-TGF beta Receptor I antibody (ab31013) This image is courtesy of an anonymous AbreviewAnti-TGF beta Receptor I antibody (ab31013) at 1/1000 dilution (for 18 hours at 4°C) + Mouse intestine whole tissue lysate at 15 µg
Secondary
An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/8000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 1 minuteBlocking Step: 5% milk for 1 hour at 4°C
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Western blot - Anti-TGF beta Receptor I antibody (ab31013)Anti-TGF beta Receptor I antibody (ab31013) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) whole cell lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
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Western blot - Anti-TGF beta Receptor I antibody (ab31013) This image is courtesy of an anonymous AbreviewAnti-TGF beta Receptor I antibody (ab31013) at 1/750 dilution (for 16 hours at 4°C) + Rat intestine whole tissue lysate at 20 µg
Secondary
An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 2 minutesBlocking Step: 5% milk for 1 hour at 20°C
