Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FBP1 antibody [EPR4620] (ab109732)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4620] to FBP1
  • Suitable for: ICC/IF, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-FBP1 antibody [EPR4620]
    See all FBP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4620] to FBP1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human stomach, Human fetal liver, Human kidney, Mouse liver, Mouse kidney, Rat liver, and MCF7 lysatesIF/ICC: HeLa cell lineFlow Cyt: MCF7 cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-FBP1 antibody [EPR4620] (ab109732)
    Immunocytochemistry/ Immunofluorescence - Anti-FBP1 antibody [EPR4620] (ab109732)
    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling FBP1 with purified ab109732 at 1:50 dilution (7.9 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Western blot - Anti-FBP1 antibody [EPR4620] (ab109732)
    Western blot - Anti-FBP1 antibody [EPR4620] (ab109732)
    All lanes : Anti-FBP1 antibody [EPR4620] (ab109732) at 1/1000 dilution (Purified)

    Lane 1 : Human kidney lysates
    Lane 2 : Mouse liver lysates
    Lane 3 : Mouse kidney lysates
    Lane 4 : Rat liver lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 37 kDa
    Observed band size: 36 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-FBP1 antibody [EPR4620] (ab109732)
    Western blot - Anti-FBP1 antibody [EPR4620] (ab109732)
    Anti-FBP1 antibody [EPR4620] (ab109732) at 1/50000 dilution + MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 37 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?

  • Flow Cytometry - Anti-FBP1 antibody [EPR4620] (ab109732)
    Flow Cytometry - Anti-FBP1 antibody [EPR4620] (ab109732)
    Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling FBP1 with purified ab109732 at 1:400 dilution (1 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunocytochemistry/ Immunofluorescence - Anti-FBP1 antibody [EPR4620] (ab109732)
    Immunocytochemistry/ Immunofluorescence - Anti-FBP1 antibody [EPR4620] (ab109732)

    ICC/IF image of ab109732 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109732, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-FBP1 antibody [EPR4620] (ab109732)
    Western blot - Anti-FBP1 antibody [EPR4620] (ab109732)
    All lanes : Anti-FBP1 antibody [EPR4620] (ab109732) at 1/1000 dilution

    Lane 1 : Human stomach lysate
    Lane 2 : Human fetal liver lysate
    Lane 3 : MCF7 lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 37 kDa

  • Anti-FBP1 antibody [EPR4620] (ab109732)
    Anti-FBP1 antibody [EPR4620] (ab109732)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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