Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸ 10 µl 127 315 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4619] to FBP1
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-FBP1 antibody [EPR4619]
See all FBP1 primary antibodies
Description

Rabbit monoclonal [EPR4619] to FBP1
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HL60 lysate treated with vitamin D3, MCF7 and Human fetal liver lysates; Human kidney and liver tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4619
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP1 antibody [EPR4619] (ab109020)

ab109020 at 1:500 staining Anti-FBP1 antibody in mouse kidney tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling FBP1 with ab109020 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP). Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-FBP1 antibody [EPR4619] (ab109020)

All lanes : Anti-FBP1 antibody [EPR4619] (ab109020) at 1/10000 dilution (purified)

Lane 1 : Mouse liver
Lane 2 : Rat liver

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 37 kDa
Observed band size: 37 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-FBP1 antibody [EPR4619] (ab109020)

All lanes : Anti-FBP1 antibody [EPR4619] (ab109020) at 1/2000 dilution (purified)

Lane 1 : MCF7 cell lysate
Lane 2 : HL-60 cell lysate treated with vitamin D3

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/2000 dilution

Predicted band size: 37 kDa
Observed band size: 37 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP1 antibody [EPR4619] (ab109020)

ab109020 at 1:500 staining Anti-FBP1 antibody in human liver tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded human liver tissue labeling FBP1 with ab109020 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP). Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-FBP1 antibody [EPR4619] (ab109020)

All lanes : Anti-FBP1 antibody [EPR4619] (ab109020) at 1/1000 dilution (unpurified)

Lane 1 : MCF7 lysate
Lane 2 : Human fetal liver lysate
Lane 3 : HL60 lysate, treated with vitamin D3

Lysates/proteins at 10 µg per lane.

Predicted band size: 37 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP1 antibody [EPR4619] (ab109020)

Unpurified ab109020 at 1/50 dilution staining FBP1 in Human Kidney by immunohistochemistry, paraffin-embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP1 antibody [EPR4619] (ab109020)

Unpurified ab109020 at 1/50 dilution staining FBP1 in Human liver by immunohistochemistry, paraffin-embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-FBP1 antibody [EPR4619] (ab109020)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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