Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FBP1 antibody [EPR4619] (ab109020)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4619] to FBP1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-FBP1 antibody [EPR4619]
    See all FBP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4619] to FBP1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HL60 lysate treated with vitamin D3, MCF7 and Human fetal liver lysates; Human kidney and liver tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP1 antibody [EPR4619] (ab109020)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP1 antibody [EPR4619] (ab109020)

    ab109020 at 1:500 staining Anti-FBP1 antibody in mouse kidney tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling FBP1 with ab109020 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP). Counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-FBP1 antibody [EPR4619] (ab109020)
    Western blot - Anti-FBP1 antibody [EPR4619] (ab109020)
    All lanes : Anti-FBP1 antibody [EPR4619] (ab109020) at 1/10000 dilution (purified)

    Lane 1 : Mouse liver
    Lane 2 : Rat liver

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-FBP1 antibody [EPR4619] (ab109020)
    Western blot - Anti-FBP1 antibody [EPR4619] (ab109020)
    All lanes : Anti-FBP1 antibody [EPR4619] (ab109020) at 1/2000 dilution (purified)

    Lane 1 : MCF7 cell lysate
    Lane 2 : HL-60 cell lysate treated with vitamin D3

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/2000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP1 antibody [EPR4619] (ab109020)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP1 antibody [EPR4619] (ab109020)

    ab109020 at 1:500 staining Anti-FBP1 antibody in human liver tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded human liver tissue labeling FBP1 with ab109020 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP). Counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-FBP1 antibody [EPR4619] (ab109020)
    Western blot - Anti-FBP1 antibody [EPR4619] (ab109020)
    All lanes : Anti-FBP1 antibody [EPR4619] (ab109020) at 1/1000 dilution (unpurified)

    Lane 1 : MCF7 lysate
    Lane 2 : Human fetal liver lysate
    Lane 3 : HL60 lysate, treated with vitamin D3

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 37 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP1 antibody [EPR4619] (ab109020)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP1 antibody [EPR4619] (ab109020)

    Unpurified ab109020 at 1/50 dilution staining FBP1 in Human Kidney by immunohistochemistry, paraffin-embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP1 antibody [EPR4619] (ab109020)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP1 antibody [EPR4619] (ab109020)

    Unpurified ab109020 at 1/50 dilution staining FBP1 in Human liver by immunohistochemistry, paraffin-embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-FBP1 antibody [EPR4619] (ab109020)
    Anti-FBP1 antibody [EPR4619] (ab109020)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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