Anti-Marapsin1 antibody [107] (ab275632)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [107] to Marapsin1
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-Marapsin1 antibody [107] -
Description
Rabbit monoclonal [107] to Marapsin1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human -
Immunogen
Recombinant full length protein (His-tag) corresponding to Human Marapsin1 aa 1-290 (C terminal).
Database link: Q9BQR3 -
Positive control
- WB: K562 whole cell lysate. IP: K562 whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 100% PBS
0.2 µm filtered solution -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
107 -
Isotype
IgG -
Research areas
Images
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Marapsin1 was immunoprecipitated from 0.5 mg of K562 (human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate with 2 µL of ab275632 and 15 µL of 50% Protein G agarose. Western blot was performed from the immunoprecipitate using ab275632 at 1/200 dilution. Rabbit IgG (H+L) Dylight 800-labeled, was used as secondary antibody at 1/5000 dilution.
Lane 1: ab275632 IP in K562 lysate
Developed using the Odssey technique.
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Anti-Marapsin1 antibody [107] (ab275632) at 1/500 dilution + K562 (human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 30 µg
Secondary
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution
Performed under reducing conditions.Developed using the Odyssey technique.
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