Anti-Endo G antibody [EP1665Y] (ab76122)
![Anti-Endo G antibody [EP1665Y] (ab76122)](https://www.abcam.com/ps/products/76/ab76122/Images/ab76122-258295-anti-endo-g-antibody-ep1665y-western-blot.jpg "Anti-Endo G antibody [EP1665Y] (ab76122)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1665Y] to Endo G
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Overview
-
Product name
Anti-Endo G antibody [EP1665Y]
See all Endo G primary antibodies -
Description
Rabbit monoclonal [EP1665Y] to Endo G -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
-
Immunogen
Synthetic peptide within Human Endo G (C terminal). The exact sequence is proprietary.
-
Positive control
- HepG2 whole cell lysate (ab7900)
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1665Y -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Endo G antibody [EP1665Y] (ab76122)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Endo G HAP1 cell lysate (20 µg)
Lane 3: Mouse liver tissue lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab76122 observed at 29 kDa. Red - loading control, ab7291, observed at 52 kDa.
ab76122 was shown to specifically react with Endo G when Endo G knockout samples were used. Wild-type and Endo G knockout samples were subjected to SDS-PAGE. ab76122 and ab7291 (loading control to alpha tubulin) were diluted 1/2000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-Endo G antibody [EP1665Y] (ab76122)](https://www.abcam.com/ps/products/76/ab76122/Images/ab76122-91461-anti-endo-g-antibody-ep1665y-western-blot.jpg)
Western blot - Anti-Endo G antibody [EP1665Y] (ab76122)Anti-Endo G antibody [EP1665Y] (ab76122) at 1/2000 dilution + HepG2 cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 36 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
-
![Anti-Endo G antibody [EP1665Y] (ab76122)](https://www.abcam.com/ps/products/76/ab76122/Images/ab76122-3-benefits-of-recombinant-antibodies.png)
Anti-Endo G antibody [EP1665Y] (ab76122)
