Paraffin-embedded human pancreas tissue stained for TGF beta Receptor I using ab235178 at 1/100 dilution in immunohistochemical analysis.

A: H&E; B & C: ab235178.

All lanes : Anti-TGF beta Receptor I antibody (ab235178) at 1/1000 dilution

Lane 1 : MW ladder
Lane 2 : TGF beta Receptor I/ALK-5 overexpressing HEK-293 (human epithelial cell line from embryonic kidney) lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 5 : Mouse liver lysate

Secondary
All lanes : Anti-RABBIT IgG (H&L) (GOAT)-HRP Antibody

Predicted band size: 55 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?


Exposure time: 3 seconds

Flow cytometric analysis of MCF7 (human breast adenocarcinoma cell line) cells labeling TGF beta Receptor I with ab235178 at 5 µl in 100 µl FACS buffer (red) compared with a secondary only control (blue).

Secondary antibody:  Donkey anti-Rabbit DyLight488 at 1/400 for 30 minutes at room temperature.