Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604)
![Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604)](https://www.abcam.com/ps/products/205/ab205604/Images/ab205604-262130-anti-tgf-beta-1-tgf-beta-3-antibody-epr19867-western-blot.jpg "Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19867] to TGF beta 1 + TGF beta 3
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Overview
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Product name
Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867]
See all TGF beta 1+TGF beta 3 primary antibodies -
Description
Rabbit monoclonal [EPR19867] to TGF beta 1 + TGF beta 3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHumanRecombinant fragment
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A549, K562, HL-60, L-929, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human fetal spleen, mouse spleen and rat spleen lysates; Recombinant protein fragment human TGF beta 1 and Recombinant protein fragment human TGF beta 3.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19867 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604)All lanes : Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604) at 1/1000 dilution
Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 3 : HL-60 (Human promyelocytic leukemia cell line) whole cell lysate
Lane 4 : L-929 (Mouse connective tissue fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 44 kDa
Observed band size: 12.5,44 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1-3: 30 seconds; Lane 4: 3 minutes.
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![Western blot - Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604)](https://www.abcam.com/ps/products/205/ab205604/Images/ab205604-262129-anti-tgf-beta-1-tgf-beta-3-antibody-epr19867-western-blot.jpg)
Western blot - Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604)Lane 1 : Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604) at 1/5000 dilution
Lanes 2-3 : Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604) at 1/1000 dilution
Lane 1 : Human fetal spleen lysate
Lane 2 : Rat spleen lysate
Lane 3 : Mouse spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lane 1 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 44 kDa
Observed band size: 12.5,44 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The cleaved form observed is consistent with what has been described in the literature (PMID:12717387; PMID:2139036; PMID:3208746).
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![Western blot - Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604)](https://www.abcam.com/ps/products/205/ab205604/Images/ab205604-262128-anti-tgf-beta-1-tgf-beta-3-antibody-epr19867-western-blot.jpg)
Western blot - Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604)All lanes : Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 44 kDa
Observed band size: 12.5,44 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
The cleaved form observed is consistent with what has been described in the literature (PMID:12717387; PMID:2139036; PMID:3208746).
-
![Western blot - Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604)](https://www.abcam.com/ps/products/205/ab205604/Images/ab205604-262127-anti-tgf-beta-1-tgf-beta-3-antibody-epr19867-western-blot.jpg)
Western blot - Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604)Lane 1 : Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604) at 1/10000 dilution
Lanes 2-3 : Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604) at 1/1000 dilution
Lane 1 : Recombinant protein fragment human TGF beta 1
Lane 2 : Recombinant protein fragment human TGF beta 2
Lane 3 : Recombinant protein fragment human TGF beta 3
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1/2: 3 minutes; Lane 3: 15 seconds.
Recombinant protein fragment human TGF beta 1 contains aa279-390 with a GST-tag. Recombinant protein fragment human TGF beta 2 contains aa303-414 with a GST-tag Recombinant protein fragment human TGF beta 3 contains aa301-412 with a GST-tag. These three fragment recombinant proteins were made in-house
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![Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604)](https://www.abcam.com/ps/products/205/ab205604/Images/ab205604-6-benefits-of-recombinant-antibodies.png)
Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604)