Anti-TGF beta 1 antibody (ab92486)
Key features and details
- Rabbit polyclonal to TGF beta 1
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-TGF beta 1 antibody
See all TGF beta 1 primary antibodies -
Description
Rabbit polyclonal to TGF beta 1 -
Host species
Rabbit -
Specificity
Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.
ab92486 detects the mature secreted form of the protein at 13 kDa. Our internal testing data suggests that this does not detect the full length protein at 44kDa. As cell lysates are unsuitable for detection of the mature form, tissue lysates should be used when using this antibody.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide corresponding to Human TGF beta 1. Surrounding amino acid 374.
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Positive control
- WB: Human spleen and kidney tissue lysates.
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General notes
This product is manufactured by BioVision, an Abcam company and was previously called 5559 Anti-TGF-ß1 Polyclonal Antibody. 5559-100 is the same size as the 100 µg size of ab92486.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.5% BSA, 30% Glycerol (glycerin, glycerine), 0.15% EDTA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TGF beta 1 antibody (ab92486)All lanes : Anti-TGF beta 1 antibody (ab92486)
Lane 1 : Human recombinant TGF-ß1 at 0.0005 µg
Lane 2 : Human kidney tissue at 50 µg
Lane 3 : Human spleen tissue at 50 µg
Predicted band size: 44 kDa
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Western blot - Anti-TGF beta 1 antibody (ab92486)All lanes : Anti-TGF beta 1 antibody (ab92486) at 5 µg/ml
Lane 1 : Human spleen tissue lysate
Lane 2 : Human kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab92486 observed at 15 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab92486 was shown to react with TGF beta 1 in western blot. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab92486 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at 5 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
