Anti-TGF beta 1 antibody (ab9758)
Key features and details
- Rabbit polyclonal to TGF beta 1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TGF beta 1 antibody
See all TGF beta 1 primary antibodies -
Description
Rabbit polyclonal to TGF beta 1 -
Host species
Rabbit -
Specificity
Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human TGF beta 1.
Sequence:FSSTEKN-C
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TGF beta 1 antibody (ab9758)All lanes : Anti-TGF beta 1 antibody (ab9758) at 1/200 dilution
Lane 1 : Reduced human recombinant TGF beta 1
Lane 2 : Non-reduced human recombinant TGF beta 1
Lysates/proteins at 0.25 µg per lane.
Secondary
All lanes : Peroxidase-conjugated anti-rabbit IgG at 1/40000 dilution
Observed band size: 12,25 kDa why is the actual band size different from the predicted?Incubated with the primary antibody overnight at 4°C.
Incubated with the secondary antibody for 30 min at room temperature.
