Anti-PAX8 antibody (ab97477)
Key features and details
- Rabbit polyclonal to PAX8
- Suitable for: WB, ICC/IF, ChIP, IHC-P, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PAX8 antibody
See all PAX8 primary antibodies -
Description
Rabbit polyclonal to PAX8 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ChIP HumanICC/IF HumanIHC-P MouseRatHumanIP HumanWB Human
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Immunogen
Recombinant protein fragment corresponding to a region within amino acids 1 and 214 of PAX8 (Uniprot: Q06710).
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Positive control
- ChIP: HEK-293T cells; WB: HEK-293T whole cell lysate; IHC-P: DLD1 xenograft, mouse liver and rat thyroid gland tissue; IF: A549 whole cells. IP: HEK-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP - Anti-PAX8 antibody (ab97477)
PAX8 antibody immunoprecipitates PAX8 protein-DNA in ChIP experiments.
ChIP Sample: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate;
A. 5 μg preimmune rabbit IgG;
B. 5 μg of ab97477.
The precipitated DNA was detected by PCR with primer set targeting to E2F1 promoter.
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Western blot - Anti-PAX8 antibody (ab97477)All lanes : Anti-PAX8 antibody (ab97477) at 1/10000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : DDDDK-tagged PAX8 transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 48 kDa10% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab97477)Immunohistochemical analysis of paraffin-embedded DLD1 xenograft tissue staining PAX8 protein at nucleolus with ab97477 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody (ab97477)Immunofluorescence analysis of paraformaldehyde-fixed A549 (human lung carcinoma cell line) whole cells lableing PAX8 with ab97477 at 1/200 dilution. Bottom: Costained with DNA probe stain.
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Immunoprecipitation - Anti-PAX8 antibody (ab97477)ab97477 immunoprecipitates PAX8 protein in IP experiments.
IP Sample: 1000 μg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
1- 40 μg HEK-293T whole cell lysate;
2- Control with 2 μg of preimmune rabbit IgG;
3- Immunoprecipitation of PAX8 protein by 2 μg ofab97477.
10% SDS-PAGE
The immunoprecipitated PAX8 protein was detected byab97477 at 1/1000 dilution, followed by anti-rabbit IgG antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab97477)Immunohistochemical analysis of paraffin-embedded mouse liver tissue staining PAX8 protein at nucleus with ab97477 at 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Western blot - Anti-PAX8 antibody (ab97477)All lanes : Anti-PAX8 antibody (ab97477) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HEK-293T cell nuclear extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 48 kDa10% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab97477)Immunohistochemical analysis of paraffin-embedded rat thyroid gland tissue staining PAX8 protein at cytosol with ab97477 at 1/500 dilution.
Antigen Retrieval:EDTA based buffer, pH 8.0, 15 min.
