Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-PAX8 antibody [EPR18715] (ab191870)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18715] to PAX8
Suitable for: IHC-Fr, WB, IHC-P, ICC/IF
Reacts with: Mouse, Human

Product name

Anti-PAX8 antibody [EPR18715]
See all PAX8 primary antibodies
Description

Rabbit monoclonal [EPR18715] to PAX8
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human thyroid cancer lysate; Human fetal kidney lysate; NIH:OVCAR-3 and SK-OV-3 whole cell lysates. IHC-P: Human thyroid carcinoma and endometrium carcinoma tissues. ICC/IF: NIH:OVCAR-3 and SK-OV-3 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18715
Isotype

IgG
Research areas

Immunohistochemistry (Frozen sections) - Anti-PAX8 antibody [EPR18715] (ab191870)

Immunohistochemistry (Frozen sections) analysis of mouse kidney tissue labelling PAX8 with ab191870 at a dilution of 1/500. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1000). Nuclear staining is visible in the mouse kidney.
Western blot - Anti-PAX8 antibody [EPR18715] (ab191870)

All lanes : Anti-PAX8 antibody [EPR18715] (ab191870) at 1/1000 dilution

Lane 1 : Human thyroid cancer lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human fetal heart lysate
Lane 4 : Human fetal kidney lysate
Lane 5 : Human fetal spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 48 kDa
Observed band size: 48 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
PAX8 is expressed in the excretory system and thyroid gland. (PMID: 1723950, 9590297, 1069301)
Western blot - Anti-PAX8 antibody [EPR18715] (ab191870)

All lanes : Anti-PAX8 antibody [EPR18715] (ab191870) at 1/1000 dilution

Lane 1 : NIH:OVCAR-3 (Human ovary adenocarcinoma cell line) whole cell lysate
Lane 2 : SK-OV-3 (Human ovarian cancer cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution

Predicted band size: 48 kDa
Observed band size: 48 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR18715] (ab191870)

Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling PAX8 with ab191870 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on tumor cells of thyroid carcinoma is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR18715] (ab191870)

Immunohistochemical analysis of paraffin-embedded Human endometrium carcinoma tissue labeling PAX8 with ab191870 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on tumor cells of the endometrium carcinoma is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [EPR18715] (ab191870)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH:OVCAR-3 (Human ovary adenocarcinoma cell line) cells labeling PAX8 with ab191870 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nucleus staining on NIH:OVCAR-3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab191870 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [EPR18715] (ab191870)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-OV-3 (Human ovarian cancer cell line) cells labeling PAX8 with ab191870 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nucleus staining on SK-OV-3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab191870 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Anti-PAX8 antibody [EPR18715] (ab191870)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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