Anti-PAX8 antibody (ab13611)
Key features and details
- Goat polyclonal to PAX8
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PAX8 antibody
See all PAX8 primary antibodies -
Description
Goat polyclonal to PAX8 -
Host species
Goat -
Specificity
This antibody is expected to recognise all five human isoforms (as represented by NP_003457; NP_039245; NP_039246; NP_039247; NP_054698). This antibody detects a strong band at approx 40kDa in Human Liver lysates. The identity of this band is unclear given the existence of 5 isoforms of PAX8 between 450 aa and 287 aa (54-34 kD). It has shown to be specific for PAX8 in experiments with nuclear extracts of PC Cl3 thyroid cells cultured for 7 days without stimulation from serum and thyrotropin (starved cells) and then stimulated with thyrotropin (TSH) for 24 h (TSH stimulated cells). A band representing PAX8 is seen in the stimulated cells at 60kD (although other non-specific background bands are also seen). We have no confirmation of which isoform this represents. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog
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Immunogen
Synthetic peptide: PHNSIRSGHGGLN, corresponding to N terminal amino acids 2-14 of Human PAX8.
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Positive control
- IHC-P: Human thyroid tissue WB: Human kidney tissue lysate, HeLa cell lysate and Human thyroid cells
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General notes
GenBank Accession Number - NP_003457; NP_039245; NP_039246; NP_039247; NP_054698
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab13611)
ab13611 (2µg/ml) staining of paraffin embedded Human Human Thyroid Gland following steamed antigen retrieval with citrate buffer pH 6 and HRP-staining shows nuclear stains in the epithelial cells around the follicles.
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Western blot - Anti-PAX8 antibody (ab13611)All lanes : Anti-PAX8 antibody (ab13611) at 1 µg/ml
Lane 1 : Human Kidney tissue lysate (35µg protein in RIPA buffer).
Lane 2 : HeLa cell lysate (35µg protein in RIPA buffer).
Predicted band size: 48 kDaDetected by chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab13611)ab13611 at 3µg/ml staining PAX8 in human thyroid gland sections by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue underwent antigen retrieval by microwave in Tris/EDTA buffer pH 9.0. The HRP-staining procedure was used for detection.
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Western blot - Anti-PAX8 antibody (ab13611)Nuclear extract of PC Cl3 thyroid cells (15
µ PAX8 is seen to be correctly regulated in panel A. Panel B shows an extended view of the same gel with other visible background bands.
The indicated PAX8 band is at 60 kD. (In some gels PAX8 runs at 55-58kD).
This image was kindly supplied as part of the review submitted by Dr Pilar Santisteban.
Nuclear extract of PC Cl3 thyroid cells (15 µg) cultured during 7 days without stimulation from serum and thyrotropin (starved cells) and then stimulated with thyrotropin (TSH) 0.5 mUI/ml during 24 h (TSH stimulated cells). PAX8 is seen to be correctly regulated in panel A. Panel B shows an extended view of the same gel with other visible background bands. The indicated PAX8 band is at
