Anti-AGO3 antibody (ab3593)
Key features and details
- Rabbit polyclonal to AGO3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-AGO3 antibody
See all AGO3 primary antibodies -
Description
Rabbit polyclonal to AGO3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human
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Immunogen
Synthetic peptide corresponding to Human AGO3 aa 350-450.
(Peptide available asab24437) -
General notes
Protein: NP_079128 (human isoform a); NP_803171 (human isoform b); NP_700451 (mouse).This product was previously labelled as Argonaute 3 / eIF2C 3
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab3593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa). ICC/IF 1/200. Target
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Function
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. -
Sequence similarities
Belongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
Cellular localization
Cytoplasm > P-body. - Information by UniProt
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Database links
- Entrez Gene: 192669 Human
- Entrez Gene: 214150 Mouse
- Entrez Gene: 313593 Rat
- Omim: 607355 Human
- SwissProt: Q9H9G7 Human
- SwissProt: Q8CJF9 Mouse
- Unigene: 657659 Human
- Unigene: 440915 Mouse
see all -
Alternative names
- 5730550L01Rik antibody
- Ago 3 antibody
- Ago3 antibody
see all
Images
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Western blot - Anti-AGO3 antibody (ab3593)All lanes : Anti-AGO3 antibody (ab3593) at 2 µg/ml
Lane 1 : Dorsal Root Ganglia (Rat) Tissue Lysate
Lane 2 : Dorsal Root Ganglia (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 97 kDa
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-AGO3 antibody (ab3593)Rat dorsal root ganglia were fixed in paraformaldehyde, blocked with 2% BSA for 30 minutes then incubated with ab3593 (1/200 dilution). For further details please refer to Hengst U et al (2006).
Protocols
Datasheets and documents
References (1)
ab3593 has been referenced in 1 publication.
- Hengst U et al. Functional and selective RNA interference in developing axons and growth cones. J Neurosci 26:5727-32 (2006). PubMed: 16723529
Images
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Western blot - Anti-AGO3 antibody (ab3593)All lanes : Anti-AGO3 antibody (ab3593) at 2 µg/ml
Lane 1 : Dorsal Root Ganglia (Rat) Tissue Lysate
Lane 2 : Dorsal Root Ganglia (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 97 kDa
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
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Immunocytochemistry/ Immunofluorescence - Anti-AGO3 antibody (ab3593)
Rat dorsal root ganglia were fixed in paraformaldehyde, blocked with 2% BSA for 30 minutes then incubated with ab3593 (1/200 dilution). For further details please refer to Hengst U et al (2006).
