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Human CD16 ELISA Kit (ab277469)

Key features and details

  • Sensitivity: 1.35 ng/ml
  • Range: 1.311 ng/ml - 320 ng/ml
  • Sample type: Cell culture supernatant, Cit plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human CD16 ELISA Kit

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall
    Inter-assay
    Sample n Mean SD CV%
    Overall

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    1.35 ng/ml

  • Range

    1.311 ng/ml - 320 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 87.7 71% - 107%
    Plasma 72.12 70% - 76%
    Cell culture media 84.86 71% - 96%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human CD16 ELISA Kit (ab277469) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD16 in serum (human CD16 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma (Collect plasma using Citrate and Heparin as an anticoagulant. EDTA is not recommended as an anticoagulant for use in this assay), and cell culture supernatants.


    This assay employs an antibody specific for Human CD16 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human CD16 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human CD16 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human CD16 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent 1 x 15ml
    Biotinylated Anti-Human CD16 Antibody 2 vials
    Anti-Human CD16 coated Microplate 1 unit
    Human CD16 standard protein (Lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml

  • Research areas

  • Function

    Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

  • Tissue specificity

    Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.

  • Sequence similarities

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

  • Post-translational
    modifications

    Glycosylated. Contains high mannose- and complex-type oligosaccharides.
    The soluble form is produced by a proteolytic cleavage.

  • Cellular localization

    Cell membrane. Secreted. Exists also as a soluble receptor.
  • Information by UniProt

  • Alternative names

    • CD 16
    • CD 16a
    • CD16
    • CD16a
    • CD16a antigen
    • CD16B
    • CD16b antigen
    • Fc fragment of IgG
    • Fc fragment of IgG low affinity IIIa receptor
    • Fc fragment of IgG low affinity IIIa receptor (CD16)
    • Fc fragment of IgG receptor IIIa
    • Fc fragment of IgG, low affinity III, receptor (CD16)
    • Fc fragment of IgG, low affinity III, receptor for (CD16)
    • Fc fragment of IgG, low affinity IIIa, receptor (CD16)
    • Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
    • Fc fragment of IgG, low affinity IIIa, receptor for
    • Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
    • Fc fragment of IgG, low affinity IIIb, receptor for (CD16)
    • Fc gamma R3
    • Fc gamma receptor III 2 (CD 16)
    • Fc gamma receptor III A
    • Fc gamma receptor IIIA
    • Fc gamma receptor IIIb (CD 16)
    • Fc gamma RIII
    • Fc gamma RIII alpha
    • Fc gamma RIII beta
    • Fc gamma RIIIa
    • Fc gamma RIIIb
    • Fc of IgG
    • Fc-gamma receptor III2 (CD 16)
    • Fc-gamma receptor III2 (CD16)
    • Fc-gamma receptor IIIb (CD16)
    • Fc-gamma RIII
    • Fc-gamma RIII-alpha
    • Fc-gamma RIIIa
    • FCG 3
    • FCG3
    • FCG3A_HUMAN
    • FCgammaRIIIA
    • FCGR 3
    • FCGR 3A
    • FCGR3
    • FCGR3A
    • FCGR3A protein
    • FCGRIII
    • FCGRIII-2
    • FcR 10
    • FcR-10
    • FcR10
    • FcRIII
    • FcRIIIa
    • IGFR 3
    • IGFR3
    • IgG Fc receptor III 1
    • IgG Fc receptor III 2
    • IgG Fc receptor III-2
    • IMD20
    • immunoglobulin G Fc receptor III
    • immunoglobulin G Fc receptor III-2
    • Low affinity IIIa receptor
    • Low affinity immunoglobulin gamma Fc region receptor III A
    • Low affinity immunoglobulin gamma Fc region receptor III-A
    • Low affinity immunoglobulin gamma Fc region receptor IIIB
    • neutrophil-specific antigen NA
    see all

  • Database links

    Images

    • Example data
      Example data

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