Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Drosha antibody [EPR12794] (ab183732)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12794] to Drosha
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Drosha antibody [EPR12794]
    See all Drosha primary antibodies

  • Description

    Rabbit monoclonal [EPR12794] to Drosha

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat, HEK-293T and HeLa cell lysates. IHC-P: Human lung adenocarcinoma tissue. Flow Cyt: Jurkat cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Drosha antibody [EPR12794] (ab183732)
    Western blot - Anti-Drosha antibody [EPR12794] (ab183732)
    All lanes : Anti-Drosha antibody [EPR12794] (ab183732) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : DROSHA knockout HEK-293T cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 159 kDa
    Observed band size: 159 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab183732 observed at 159 kDa. Red - loading control, ab8245 observed at 37 kDa.

    ab183732 Anti-Drosha antibody [EPR12794] was shown to specifically react with Drosha in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266217 (knockout cell lysate ab257171) was used. Wild-type and Drosha knockout samples were subjected to SDS-PAGE. ab183732 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-Drosha antibody [EPR12794] (ab183732)
    Western blot - Anti-Drosha antibody [EPR12794] (ab183732)
    All lanes : Anti-Drosha antibody [EPR12794] (ab183732) at 1/50000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 159 kDa
    Observed band size: 159 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Drosha antibody [EPR12794] (ab183732)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Drosha antibody [EPR12794] (ab183732)

    Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling Drosha with ab183732 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-Drosha antibody [EPR12794] (ab183732)
    Flow Cytometry - Anti-Drosha antibody [EPR12794] (ab183732)

    Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling Drosha with purified ab183732 at 1/230 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

  • Anti-Drosha antibody [EPR12794] (ab183732)
    Anti-Drosha antibody [EPR12794] (ab183732)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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