Anti-Argonaute-2 antibody (ab32381)
Key features and details
- Rabbit polyclonal to Argonaute-2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Argonaute-2 antibody
See all Argonaute-2 primary antibodies -
Description
Rabbit polyclonal to Argonaute-2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow
Does not react with:
Drosophila melanogaster -
Immunogen
Synthetic peptide corresponding to Argonaute-2 aa 350-450 conjugated to keyhole limpet haemocyanin.
(Peptide available asab32380) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32381 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P (2) Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.WB (3) Use a concentration of 2 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 97 kDa).Notes IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.WB
Use a concentration of 2 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 97 kDa).Target
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Function
Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by EIF2C2/AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also upregulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and upregulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions. -
Sequence similarities
Belongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
Domain
The Piwi domain may perform RNA cleavage by a mechanism similar to that of RNase H. However while RNase H utilizes a triad of Asp-Asp-Glu (DDE) for metal ion coordination, this protein appears to utilize a triad of Asp-Asp-His (DDH). -
Post-translational
modificationsHydroxylated. 4-hydroxylation appears to enhance protein stability but is not required for miRNA-binding or endonuclease activity. -
Cellular localization
Cytoplasm > P-body. Nucleus. Translational repression of mRNAs results in their recruitment to P-bodies. Translocation to the nucleus requires IMP8. - Information by UniProt
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Database links
- Entrez Gene: 27161 Human
- Entrez Gene: 239528 Mouse
- Entrez Gene: 59117 Rat
- Omim: 606229 Human
- SwissProt: Q9UKV8 Human
- SwissProt: Q8CJG0 Mouse
- SwissProt: Q9QZ81 Rat
- Unigene: 449415 Human
see all -
Alternative names
- Ago 2 antibody
- AGO2_HUMAN antibody
- Argonaute 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody (ab32381)Oak et al PLoS One. 2011;6(6):e21253. doi: 10.1371/journal.pone.0021253. Epub 2011 Jun 21. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Immunohistochemical analysis of Argonaute2 (AGO2) in human tissues sections from slowly progressive, rapidly progressive, or normal biopsies.
Representative images of slowly progressive (A–B, E–F), rapidly progressive (C–D, G–H), and normal (I–J) biopsies stained with IgG control (A, C, E, G, & I) and anti-AGO2 antibody (B, D, F, H, & J) are shown. Images A, B, C, & D were stained with antibody concentrations of 20 µg/ml and images E, F, G, H, I, & J were stained with an antibody concentration of 4 µg/ml. Sections were counterstained with hematoxylin. Protein expression stains brown in this procedure (original magnification: ×200).
Panels A-D shown.
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Western blot - Anti-Argonaute-2 antibody (ab32381)All lanes : Anti-Argonaute-2 antibody (ab32381) at 2 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.The identification of the 55 kDa band is unclear but this band has also been observed in HeLa lysates in the Western Blot of ab5072, targeting Drosophila Ago2 / eIF2C2.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody (ab32381)IHC image of Argonaute-2 antibody staining in a section of formalin-fixed paraffin-embedded human breast adenocarcinoma* performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab32381, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Protocols
Datasheets and documents
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Datasheet download
References (194)
ab32381 has been referenced in 194 publications.
- Witek P et al. Effect of neonatal exposure to endocrine-active compounds on epigenetic regulation of gene expression in corpus luteum of gilts. Theriogenology 159:45-52 (2021). PubMed: 33113443
- Karvinen S et al. MicroRNAs in Extracellular Vesicles in Sweat Change in Response to Endurance Exercise. Front Physiol 11:676 (2020). PubMed: 32760282
- Teng F et al. LncRNA MYLK-AS1 facilitates tumor progression and angiogenesis by targeting miR-424-5p/E2F7 axis and activating VEGFR-2 signaling pathway in hepatocellular carcinoma. J Exp Clin Cancer Res 39:235 (2020). PubMed: 33168027
- Lin JH et al. Long non-coding RNA B4GALT1-Antisense RNA 1/microRNA-30e/SRY-box transcription factor 9 signaling axis contributes to non-small cell lung cancer cell growth. Oncol Lett 20:284 (2020). PubMed: 33014162
- Tao L et al. lncRNA CASC2 Enhances 131I Sensitivity in Papillary Thyroid Cancer by Sponging miR-155. Biomed Res Int 2020:7183629 (2020). PubMed: 33134385
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody (ab32381) Oak et al PLoS One. 2011;6(6):e21253. doi: 10.1371/journal.pone.0021253. Epub 2011 Jun 21. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Immunohistochemical analysis of Argonaute2 (AGO2) in human tissues sections from slowly progressive, rapidly progressive, or normal biopsies.
Representative images of slowly progressive (A–B, E–F), rapidly progressive (C–D, G–H), and normal (I–J) biopsies stained with IgG control (A, C, E, G, & I) and anti-AGO2 antibody (B, D, F, H, & J) are shown. Images A, B, C, & D were stained with antibody concentrations of 20 µg/ml and images E, F, G, H, I, & J were stained with an antibody concentration of 4 µg/ml. Sections were counterstained with hematoxylin. Protein expression stains brown in this procedure (original magnification: ×200).
Panels A-D shown.
-
Western blot - Anti-Argonaute-2 antibody (ab32381)All lanes : Anti-Argonaute-2 antibody (ab32381) at 2 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.The identification of the 55 kDa band is unclear but this band has also been observed in HeLa lysates in the Western Blot of ab5072, targeting Drosophila Ago2 / eIF2C2.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody (ab32381)
IHC image of Argonaute-2 antibody staining in a section of formalin-fixed paraffin-embedded human breast adenocarcinoma* performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab32381, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
