Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸ 10 µl 137 366 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Argonaute-2 antibody [EPR10410] (ab156870)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10410] to Argonaute-2
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-Argonaute-2 antibody [EPR10410]
See all Argonaute-2 primary antibodies
Description

Rabbit monoclonal [EPR10410] to Argonaute-2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human Argonaute-2 aa 1-100. The exact sequence is proprietary.
Positive control
- WB: HeLa, MCF7, HepG2 and K562 cell lysates. Rat liver and mouse liver lysates. IHC-P: Human breast carcinoma and human kidney tissues. Flow Cyt: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR10410
Isotype

IgG
Research areas

Western blot - Anti-Argonaute-2 antibody [EPR10410] (ab156870)

All lanes : Anti-Argonaute-2 antibody [EPR10410] (ab156870) at 1/5000 dilution (purified)

Lane 1 : Rat liver lysates
Lane 2 : Mouse kidney lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 97 kDa

Blocking and diluting buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10410] (ab156870)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human ovarian carcinoma tissue sections labeling Argonaute -2 with purified ab156870 at 1:100 dilution (1.9 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-Argonaute-2 antibody [EPR10410] (ab156870)

All lanes : Anti-Argonaute-2 antibody [EPR10410] (ab156870) at 1/1000 dilution (purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HUVEC (Human umbilical vein endothelial cell) whole cell lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 97 kDa

Blocking and diluting buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [EPR10410] (ab156870)

Immunocytochemistry/ Immunofluorescence analysis of MCF-7 (Human breast adenocarcinoma epithelial cell) cells labeling Argonaute-2 with Purified ab156870 at 1:200 dilution (9.5μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10410] (ab156870)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Ago2 / eIF2C2 with unpurified ab156870 at 1/50 dilution.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Western blot - Anti-Argonaute-2 antibody [EPR10410] (ab156870)

All lanes : Anti-Argonaute-2 antibody [EPR10410] (ab156870) at 1/1000 dilution (unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : K562 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 97 kDa
Flow Cytometry - Anti-Argonaute-2 antibody [EPR10410] (ab156870)

Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Argonaute-2 (red) with unpurified ab156870 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10410] (ab156870)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Ago2 / eIF2C2 with unpurified ab156870 at 1/50 dilution.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Anti-Argonaute-2 antibody [EPR10410] (ab156870)

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