Anti-Argonaute-2 antibody [11A9] - BSA and Azide free (ab255768)
![Anti-Argonaute-2 antibody [11A9] - BSA and Azide free (ab255768)](https://www.abcam.com/ps/products/255/ab255768/Images/ab255768-369712-anti-argonaute-2-antibody-11a9-western-blot-human.jpg "Anti-Argonaute-2 antibody [11A9] - BSA and Azide free (ab255768)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [11A9] to Argonaute-2 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Argonaute-2 antibody [11A9] - BSA and Azide free
See all Argonaute-2 primary antibodies -
Description
Rat monoclonal [11A9] to Argonaute-2 - BSA and Azide free -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.
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Positive control
- WB: HeLa, NCI-H1299, HCT 116 and A549 whole cell lysates.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab255768 is the carrier-free version of ab252812. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
11A9 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-Argonaute-2 antibody [11A9] - BSA and Azide free (ab255768)All lanes : Anti-Argonaute-2 antibody [11A9] (ab252812) at 0.49 µg/ml
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : NCI-H1299 (human lung carcinoma epithelial cell), whole cell lysate
Lane 3 : HCT 116 (human colorectal carcinoma epithelial cell), whole cell lysate
Lane 4 : A549 (Human lung carcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 97 kDa
Observed band size: 97 kDaBlocking abd dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
The molecular weight is consistent with the benchmark: ab186733. The positive cells were the consistent with the literature provided by licensor (PMID: 1843089).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252812).
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![Anti-Argonaute-2 antibody [11A9] - BSA and Azide free (ab255768)](https://www.abcam.com/ps/products/255/ab255768/Images/ab255768-2-benefits-of-recombinant-antibodies.png)
Anti-Argonaute-2 antibody [11A9] - BSA and Azide free (ab255768)
