Antibodies, Proteins, Kits and Reagents for Life Science

331 689 ₸ Product size

100 µl 331 689 ₸ 10 µl 137 366 ₸

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Anti-Argonaute-2 antibody [EPR10411] (ab186733)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10411] to Argonaute-2
Suitable for: ICC/IF, Flow Cyt, IP, WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Argonaute-2 antibody [EPR10411]
See all Argonaute-2 primary antibodies
Description

Rabbit monoclonal [EPR10411] to Argonaute-2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Human
IP	Human
WB	Rat Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa, MCF7, HepG2, C6 and RAW 264.7 cell lysates; Human cervix carcinoma and Mouse kidney tissues; HeLa and MCF7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR10411
Isotype

IgG
Research areas

Western blot - Anti-Argonaute-2 antibody [EPR10411] (ab186733)

All lanes : Anti-Argonaute-2 antibody [EPR10411] (ab186733) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 97 kDa
Observed band size: 97 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10411] (ab186733)

Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Ago2 / eIF2C2 with ab186733 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [EPR10411] (ab186733)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Ago2 / eIF2C2 with ab186733 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10411] (ab186733)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Ago2 / eIF2C2 with ab186733 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-Argonaute-2 antibody [EPR10411] (ab186733)

All lanes : Anti-Argonaute-2 antibody [EPR10411] (ab186733) at 1/1000 dilution

Lane 1 : C6 cell lysate
Lane 2 : RAW 264.7 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 97 kDa
Observed band size: 97 kDa
Flow Cytometry - Anti-Argonaute-2 antibody [EPR10411] (ab186733)

Flow cytometric analysis of 2% paraformaldehyde-fixed MCF7 cells labeling Ago2 / eIF2C2 with ab186733 at 1/60 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Immunoprecipitation - Anti-Argonaute-2 antibody [EPR10411] (ab186733)

Western blot analysis of Ago2 / eIF2C2 in MCF7 cell lysate immunoprecipitated with ab186733 at 1/50 dilution.
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Anti-Argonaute-2 antibody [EPR10411] (ab186733)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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