Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)
![Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)](https://www.abcam.com/ps/products/57/ab57113/Images/ab57113-368598-anti-argonaute-2-antibody-2e12-1c9-chip-grade-western-blot-MCF7-lysate.jpg "Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)")
Key features and details
- Mouse monoclonal [2E12-1C9] to Argonaute-2
- Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-Argonaute-2 antibody [2E12-1C9]
See all Argonaute-2 primary antibodies -
Description
Mouse monoclonal [2E12-1C9] to Argonaute-2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB HumanRecombinant fragment
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Immunogen
Recombinant fragment corresponding to Human Argonaute-2 aa 483-859.
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Positive control
- IHC-P: Human stomach. Flow cyt: HeLa cells. WB: HEK-293 cell.
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General notes
This product was changed from ascites to tissue culture supernatant on 30th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Constituent: PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
2E12-1C9 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)Anti-Argonaute-2 antibody [2E12-1C9] (ab57113) + MCF7 cell lysate
Predicted band size: 97 kDa
Observed band size: 97 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)](https://www.abcam.com/ps/products/57/ab57113/Images/ab57113-261138-anti-argonaute-2-antibody-2e12-1c9-chip-grade-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)Ago2 / eIF2C2 antibody (ab57113) used in immunofluorescence at 10ug/ml on HeLa cells.
This image was generated using the ascites version of the product.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)](https://www.abcam.com/ps/products/57/ab57113/Images/ab57113-261137-anti-argonaute-2-antibody-2e12-1c9-chip-grade-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)Ago2 / eIF2C2 antibody (ab57113) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human stomach.
This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)](https://www.abcam.com/ps/products/57/ab57113/Images/ab57113-70644-anti-argonaute-2-antibody-2e12-1c9-chip-grade-flow-cytometry.jpg)
Flow Cytometry - Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)Overlay histogram showing HeLa cells stained with ab57113 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57113, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
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![Western blot - Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)](https://www.abcam.com/ps/products/57/ab57113/Images/ab57113-334180-anti-argonaute-2-antibody-2e12-1c9-chip-grade-western-blot.jpg)
Western blot - Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)All lanes : Anti-Argonaute-2 antibody [2E12-1C9] (ab57113) at 5 µg/ml
Lane 1 : Argonaute-2 overexpressing HEK-293 cells line
Lane 2 : Argonaute-2 overexpressing HEK-293 cell line cotransfected with Argonaute-2 validated chimera RNAi.
Predicted band size: 97 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?The band at about 42 kDa corresponds to the recombinant fragment of human Argonaute-2 aa 483-859 (377 aa length).
The loading control is GAPDH.
This image was generated using the ascites version of the product.
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![Western blot - Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)](https://www.abcam.com/ps/products/57/ab57113/Images/ab57113-70645-anti-argonaute-2-antibody-2e12-1c9-chip-grade-western-blot.jpg)
Western blot - Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)Western Blot detection against the recombinant fragment imunogen (68 KDa for a.a. ~377 plus GST tag +26 kDa).
This image was generated using the ascites version of the product.
