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Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)

Key features and details

  • Mouse monoclonal [2E12-1C9] to Argonaute-2
  • Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG1

Overview

  • Product name

    Anti-Argonaute-2 antibody [2E12-1C9]
    See all Argonaute-2 primary antibodies

  • Description

    Mouse monoclonal [2E12-1C9] to Argonaute-2

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human Argonaute-2 aa 483-859.

  • Positive control

    • IHC-P: Human stomach. Flow cyt: HeLa cells. WB: HEK-293 cell.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 30th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Images

  • Western blot - Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)
    Western blot - Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)
    Anti-Argonaute-2 antibody [2E12-1C9] (ab57113) + MCF7 cell lysate

    Predicted band size: 97 kDa
    Observed band size: 97 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)
    Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)

    Ago2 / eIF2C2 antibody (ab57113) used in immunofluorescence at 10ug/ml on HeLa cells.

    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)

    Ago2 / eIF2C2 antibody (ab57113) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human stomach.

    This image was generated using the ascites version of the product.

  • Flow Cytometry - Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)
    Flow Cytometry - Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)

    Overlay histogram showing HeLa cells stained with ab57113 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57113, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)
    Western blot - Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)
    All lanes : Anti-Argonaute-2 antibody [2E12-1C9] (ab57113) at 5 µg/ml

    Lane 1 : Argonaute-2 overexpressing HEK-293 cells line
    Lane 2 : Argonaute-2 overexpressing HEK-293 cell line cotransfected with Argonaute-2 validated chimera RNAi.

    Predicted band size: 97 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?



    The band at about 42 kDa corresponds to the recombinant fragment of human Argonaute-2 aa 483-859 (377 aa length).

    The loading control is GAPDH.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)
    Western blot - Anti-Argonaute-2 antibody [2E12-1C9] (ab57113)

    Western Blot detection against the recombinant fragment imunogen (68 KDa for a.a. ~377 plus GST tag +26 kDa).

    This image was generated using the ascites version of the product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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