Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ORP8 antibody [mAbcam 60110] (ab60110)

Key features and details

  • Mouse monoclonal [mAbcam 60110] to ORP8
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-ORP8 antibody [mAbcam 60110]
    See all ORP8 primary antibodies

  • Description

    Mouse monoclonal [mAbcam 60110] to ORP8

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Horse, Dog, Chimpanzee

  • Immunogen

    Synthetic peptide corresponding to Human ORP8 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab75267)

  • Positive control

    • This antibody gave a positive signal in human kidney tissue lysate and in the following whole cell lysates: HEK293; THP1.

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-ORP8 antibody [mAbcam 60110] (ab60110)
    Western blot - Anti-ORP8 antibody [mAbcam 60110] (ab60110)
    All lanes : Anti-ORP8 antibody [mAbcam 60110] (ab60110) at 5 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 101 kDa
    Observed band size: 101 kDa


    Exposure time: 8 minutes


    We recommend using 3% milk as the blocking agent in Western Blot.

  • Western blot - Anti-ORP8 antibody [mAbcam 60110] (ab60110)
    Western blot - Anti-ORP8 antibody [mAbcam 60110] (ab60110)
    All lanes : Anti-ORP8 antibody [mAbcam 60110] (ab60110) at 5 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 101 kDa
    Observed band size: 101 kDa
    Additional bands at: 44 kDa, 55 kDa, 80 kDa, 85 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds


    1% BSA was used as a blocking agent.

  • Flow Cytometry - Anti-ORP8 antibody [mAbcam 60110] (ab60110)
    Flow Cytometry - Anti-ORP8 antibody [mAbcam 60110] (ab60110)
    Overlay histogram showing HEK293 cells stained with ab60110 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60110, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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