Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Argonaute-2 antibody [EPR10410] - BSA and Azide free (ab249284)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10410] to Argonaute-2 - BSA and Azide free
Suitable for: ICC, IHC-P, WB, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-Argonaute-2 antibody [EPR10410] - BSA and Azide free
See all Argonaute-2 primary antibodies
Description

Rabbit monoclonal [EPR10410] to Argonaute-2 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: ICC, IHC-P, WB, Flow Cytmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab249284 is the carrier-free version of ab156870. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249284 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR10410
Isotype

IgG
Research areas

Western blot - Anti-Argonaute-2 antibody [EPR10410] - BSA and Azide free (ab249284)

All lanes : Anti-Argonaute-2 antibody [EPR10410] (ab156870) at 1/5000 dilution (purified)

Lane 1 : Rat liver lysates
Lane 2 : Mouse kidney lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 97 kDa

This data was developed using ab156870, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10410] - BSA and Azide free (ab249284)

This data was developed using ab156870, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human ovarian carcinoma tissue sections labeling Argonaute -2 with purified ab156870 at 1:100 dilution (1.9 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-Argonaute-2 antibody [EPR10410] - BSA and Azide free (ab249284)

All lanes : Anti-Argonaute-2 antibody [EPR10410] (ab156870) at 1/1000 dilution (purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HUVEC (Human umbilical vein endothelial cell) whole cell lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 97 kDa

This data was developed using ab156870, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Immunocytochemistry - Anti-Argonaute-2 antibody [EPR10410] - BSA and Azide free (ab249284)

This data was developed using ab156870, the same antibody clone in a different buffer formulation.Immunocytochemistry/ Immunofluorescence analysis of MCF-7 (Human breast adenocarcinoma epithelial cell) cells labeling Argonaute-2 with Purified ab156870 at 1:200 dilution (9.5μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10410] - BSA and Azide free (ab249284)

This data was developed using ab156870, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Ago2 / eIF2C2 with unpurified ab156870 at 1/50 dilution. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Western blot - Anti-Argonaute-2 antibody [EPR10410] - BSA and Azide free (ab249284)

All lanes : Anti-Argonaute-2 antibody [EPR10410] (ab156870) at 1/1000 dilution (unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : K562 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 97 kDa

This data was developed using ab156870, the same antibody clone in a different buffer formulation.
Flow Cytometry - Anti-Argonaute-2 antibody [EPR10410] - BSA and Azide free (ab249284)

This data was developed using ab156870, the same antibody clone in a different buffer formulation.Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Argonaute-2 (red) with unpurified ab156870 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10410] - BSA and Azide free (ab249284)

This data was developed using ab156870, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Ago2 / eIF2C2 with unpurified ab156870 at 1/50 dilution. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Anti-Argonaute-2 antibody [EPR10410] - BSA and Azide free (ab249284)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com