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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing RNAi

Anti-Argonaute-2 antibody [11A9] (ab252812)

Price and availability

307 566 ₸

Availability

Order now and get it on Tuesday March 23, 2021

Anti-Argonaute-2 antibody [11A9] (ab252812)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [11A9] to Argonaute-2
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-Argonaute-2 antibody [11A9]
    See all Argonaute-2 primary antibodies
  • Description

    Rat monoclonal [11A9] to Argonaute-2
  • Host species

    Rat
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WB: HeLa, NCI-H1299, HCT 116 and A549 whole cell lysates.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Ion Exchange Chromatography
  • Clonality

    Monoclonal
  • Clone number

    11A9
  • Isotype

    IgG2a
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • RNAi
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Argonaute and Piwi
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Eukaryotic Initiation factors (eIF's)
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Nuclear Pore Complex

Images

  • Western blot - Anti-Argonaute-2 antibody [11A9] (ab252812)
    Western blot - Anti-Argonaute-2 antibody [11A9] (ab252812)
    Lanes 1-2 & 4 : Anti-Argonaute-2 antibody [11A9] (ab252812) at 0.49 µg/ml
    Lane 3 : Anti-Argonaute-2 antibody [11A9] (ab252812) at 0.49 µg

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : NCI-H1299 (human lung carcinoma epithelial cell), whole cell lysate
    Lane 3 : HCT 116 (human colorectal carcinoma epithelial cell), whole cell lysate
    Lane 4 : A549 (Human lung carcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 97 kDa
    Observed band size: 97 kDa



    Blocking abd dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The molecular weight is consistent with the benchmark: ab186733. The positive cells were the consistent  with the literature provided by licensor (PMID: 1843089).

  • Anti-Argonaute-2 antibody [11A9] (ab252812)
    Anti-Argonaute-2 antibody [11A9] (ab252812)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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