Anti-Argonaute-2 (phospho Y393) antibody (ab215746)
Key features and details
- Rabbit polyclonal to Argonaute-2 (phospho Y393)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Argonaute-2 (phospho Y393) antibody
See all Argonaute-2 primary antibodies -
Description
Rabbit polyclonal to Argonaute-2 (phospho Y393) -
Host species
Rabbit -
Specificity
The site is conserved in Argonaute-1 and Argonaute-4, but not in Argonaute-3. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Argonaute-2 (phospho Y393) conjugated to keyhole limpet haemocyanin.
Database link: Q9UKV8 -
Positive control
- A431 cell lysate treated with 100 ng/mL EGF for 60 min.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 0.1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab215746 was cross-absorbed to unphospho-Argonaute-2 (phospho Y393), before affinity purification using Argonaute-2 (phospho Y393) peptide (without carrier). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Argonaute-2 (phospho Y393) antibody (ab215746)Lanes 1-2 : rat monoclonal anti-Ago2
Lanes 3-4 : Anti-Argonaute-2 (phospho Y393) antibody (ab215746) at 1/1000 dilution
Lane 1 : A431 cells treated with EGF (100 ng/ml for 60 min.)
Lanes 2 & 4 : A431 cells treated with EGF (100 ng/ml for 60 min.) treated with lambda phosphatase
Lane 3 : A431 cell lysate treated with EGF (100 ng/ml for 60 min.)
Predicted band size: 97 kDaWestern blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, and 0.04% Tween20 for 1 h at room temperature.
