Anti-KCNK18/TRESK antibody (ab106411)
Key features and details
- Rabbit polyclonal to KCNK18/TRESK
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-KCNK18/TRESK antibody -
Description
Rabbit polyclonal to KCNK18/TRESK -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat -
Immunogen
A 17 amino acid peptide from near the center of Human KCNK18/TRESK (NP_862823).
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Positive control
- Rat brain tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNK18/TRESK antibody (ab106411)ab106411, at 5 µg/ml, staining KCNK18/TRESK in paraffin embedded Rat brain tissue.
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Western blot - Anti-KCNK18/TRESK antibody (ab106411)Lane 1 : Anti-KCNK18/TRESK antibody (ab106411) at 1 µg/ml
Lane 2 : Anti-KCNK18/TRESK antibody (ab106411) at 2 µg/ml
All lanes : Rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 44 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-KCNK18/TRESK antibody (ab106411)ICC/IF image of ab106411 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab106411, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunocytochemistry/ Immunofluorescence - Anti-KCNK18/TRESK antibody (ab106411)Immunofluorescence of TRESK in Rat Brain cells using ab106411 at 20 ug/ml.
