Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AMPK gamma 1 antibody [Y308] (ab32508)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y308] to AMPK gamma 1
  • Suitable for: IP, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-AMPK gamma 1 antibody [Y308]
    See all AMPK gamma 1 primary antibodies

  • Description

    Rabbit monoclonal [Y308] to AMPK gamma 1

  • Host species

    Rabbit

  • Specificity

    ab32508 recognises 5’-AMP-activated protein kinase (AMPK).

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human AMPK gamma 1 aa 300-400 (C terminal). The exact sequence is proprietary.
    (Peptide available as ab218345)

  • Positive control

    • Jurkat whole cell lysate (ab7899). Flow Cyt: HeLa cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-AMPK gamma 1 antibody [Y308] (ab32508)
    Western blot - Anti-AMPK gamma 1 antibody [Y308] (ab32508) This image is courtesy of an anonymous Abreview
    All lanes : Anti-AMPK gamma 1 antibody [Y308] (ab32508) at 1/1000 dilution

    All lanes : HEK293 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Alexa Fluor® 690-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa


    Exposure time: 5 minutes

    See Abreview

  • Flow Cytometry - Anti-AMPK gamma 1 antibody [Y308] (ab32508)
    Flow Cytometry - Anti-AMPK gamma 1 antibody [Y308] (ab32508)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling AMPK gamma 1 with ab32508 at 1/100 dilution (1 µg) (Red). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were fixed with 4% paraformaldehyde. Rabbit monoclonal IgG (ab172730) was used as isotype control (Black). Unlabelled control: Cells without incubation with primary antibody and secondary antibody (Blue).

  • Western blot - Anti-AMPK gamma 1 antibody [Y308] (ab32508)
    Western blot - Anti-AMPK gamma 1 antibody [Y308] (ab32508)
    Anti-AMPK gamma 1 antibody [Y308] (ab32508) at 1/1000 dilution + Jurkat cell lysate

    Predicted band size: 38 kDa
    Observed band size: 38 kDa

  • Immunoprecipitation - Anti-AMPK gamma 1 antibody [Y308] (ab32508)
    Immunoprecipitation - Anti-AMPK gamma 1 antibody [Y308] (ab32508)

    AMPK gamma 1 was immunoprecipitated using 1 mg Jurkat whole cell extract, 0.2 ug of Rabbit monoclonal [Y308] to AMPK gamma 1and 50µl of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10min under agitation. No antibody was added to the control (lane 2). Jurkat whole cell extractdiluted in RIPA buffer was added to each sample and incubated for 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32508. Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697). Bands: 37kDa: AMPK gamma 1.

  • Anti-AMPK gamma 1 antibody [Y308] (ab32508)
    Anti-AMPK gamma 1 antibody [Y308] (ab32508)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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